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Literature summary for 3.2.1.B28 extracted from
- Quercetin production from rutin by a thermostable beta-rutinosidase from Pyrococcus furiosus (2012), Biotechnol. Lett., 34, 483-489.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
isoquercitrin | pH 5.0, 95°C | Pyrococcus furiosus |  |
| 3.095 | - |
quercitrin | pH 5.0, 95°C | Pyrococcus furiosus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q51723 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.23 | - |
substrate: quercitrin, pH 5.0, 95°C | Pyrococcus furiosus |
| 220 | - |
substrate: isoquercitrin, pH 5.0, 95°C | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isoquercitrin + H2O | i.e. quercetin 3-O-beta-D-glucoside | Pyrococcus furiosus | quercetin + beta-D-glucose | - |
? | |
| additional information | the enzyme shows also beta-rutinosidase activity with rutin (hydrolysis of rutin to quercetin and rutinose) | Pyrococcus furiosus | ? | - |
? | |
| quercitrin + H2O | i.e. quercetin 3-O-alpha-L-rhamnoside | Pyrococcus furiosus | quercetin + alpha-L-rhamnose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
half-life: 1017 h | Pyrococcus furiosus |
| 80 | - |
half-life: 461 h | Pyrococcus furiosus |
| 85 | - |
half-life: 286 h | Pyrococcus furiosus |
| 90 | - |
half-life: 173 h | Pyrococcus furiosus |
| 95 | - |
half-life: 101 h | Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
quercitrin | pH 5.0, 95°C | Pyrococcus furiosus | |
| 53.3 | - |
isoquercitrin | pH 5.0, 95°C | Pyrococcus furiosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Pyrococcus furiosus |
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