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Literature summary for 3.2.1.98 extracted from

  • Valk, V.; van der Kaaij, R.M.; Dijkhuizen, L.
    Characterization of the starch-acting MaAmyB enzyme from Microbacterium aurum B8.A representing the novel subfamily GH13_42 with an unusual, multi-domain organization (2016), Sci. Rep., 6, 36100 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a full length protein with both N- and C-terminal His-tags Microbacterium aurum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activity is only observed in the presence of 10 mM of CaCl2 Microbacterium aurum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Microbacterium aurum in Microbacterium aurum B8.A, the enzyme may contribute to degradation of starch granules by rapidly hydrolyzing the helical and linear starch chains that become exposed after pore formation by MaAmyA ?
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 ?
starch + H2O Microbacterium aurum B8.A in Microbacterium aurum B8.A, the enzyme may contribute to degradation of starch granules by rapidly hydrolyzing the helical and linear starch chains that become exposed after pore formation by MaAmyA ?
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 ?

Organism

Organism UniProt Comment Textmining
Microbacterium aurum A0A1B3IKE0
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-
Microbacterium aurum B8.A A0A1B3IKE0
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Microbacterium aurum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-maltoheptaoside + H2O immediate release of 4-nitrophenol Microbacterium aurum 4-nitrophenol + maltoheptaose
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 ?
4-nitrophenyl alpha-maltoheptaoside + H2O immediate release of 4-nitrophenol Microbacterium aurum B8.A 4-nitrophenol + maltoheptaose
-
 ?
granular starch + H2O incubation of granular starch with the enzyme does not result in accumulation of maltohexaose but in direct release of glucose, maltose and maltotriose Microbacterium aurum maltotriose + maltose + D-glucose
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 ?
granular starch + H2O incubation of granular starch with the enzyme does not result in accumulation of maltohexaose but in direct release of glucose, maltose and maltotriose Microbacterium aurum B8.A maltotriose + maltose + D-glucose
-
 ?
maltoheptaose + H2O
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 Microbacterium aurum maltohexaose + maltotriose + maltose + D-glucose
-
 ?
maltoheptaose + H2O
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 Microbacterium aurum B8.A maltohexaose + maltotriose + maltose + D-glucose
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 ?
additional information the enzyme is inactive on maltose Microbacterium aurum ?
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 ?
additional information the enzyme is inactive on maltose Microbacterium aurum B8.A ?
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 ?
soluble starch + H2O initial accumulation of maltohexaose, which in time is further hydrolyzed to smaller altooligosaccharides, resulting in accumulation of mostly maltotriose, maltose and glucose Microbacterium aurum maltohexaose + maltotriose + maltose + D-glucose
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 ?
starch + H2O in Microbacterium aurum B8.A, the enzyme may contribute to degradation of starch granules by rapidly hydrolyzing the helical and linear starch chains that become exposed after pore formation by MaAmyA Microbacterium aurum ?
-
 ?
starch + H2O in Microbacterium aurum B8.A, the enzyme may contribute to degradation of starch granules by rapidly hydrolyzing the helical and linear starch chains that become exposed after pore formation by MaAmyA Microbacterium aurum B8.A ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-glucan 1,4-alpha-maltohexaosidase
-
 Microbacterium aurum
MaAmyB
-
 Microbacterium aurum