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Literature summary for 3.2.1.91 extracted from
- Competitive inhibition of cellobiohydrolase I by manno-oligosaccharides (2015), Enzyme Microb. Technol., 68, 62-68 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mannan | - |
Thermoascus aurantiacus |  |
| mannobiose | strong inhibition, effect on enzyme kinetics, overview | Thermoascus aurantiacus | |
| Mannotetraose | effect on enzyme kinetics, overview | Thermoascus aurantiacus | |
| Mannotriose | effect on enzyme kinetics, overview | Thermoascus aurantiacus | |
| additional information | manno-oligosaccharides, MOS, are competitive inhibitors ofthe CBHI. The negative effects of mannan and manno-oligosaccharides (MOS) on the hydrolytic action of cellulases indicates that the inhibitory effect is at least partly attributed to the inhibition of Ta Cel7A (CBHI), but not on beta-glucosidase | Thermoascus aurantiacus | |
| xylan | both soluble and insoluble xylans inhibit the activities of CBHI | Thermoascus aurantiacus | |
| xylo-oligosaccharides | XOS, competitive inhibitors of CBHI from Thermoascus aurantiacus | Thermoascus aurantiacus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.67 | - |
4-nitrophenyl beta-D-cellobioside | pH 5.0, 50°C | Thermoascus aurantiacus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoascus aurantiacus | A7WNU2 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-cellobioside + H2O | - |
Thermoascus aurantiacus | 4-nitrophenol + D-cellobiose | - |
? | |
| avicel + H2O | microcrystalline cellulose, Avicel PH-101, at 2% | Thermoascus aurantiacus | D-glucose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBHI | - |
Thermoascus aurantiacus |
| Cel7A | - |
Thermoascus aurantiacus |
| cellobiohydrolase I | - |
Thermoascus aurantiacus |
| glucanase | - |
Thermoascus aurantiacus |
| Ta Cel7A | - |
Thermoascus aurantiacus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Thermoascus aurantiacus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Thermoascus aurantiacus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics | Thermoascus aurantiacus |
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Release 2023.1 (January 2023)
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