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Literature summary for 3.2.1.91 extracted from

  • Zhang, T.; Datta, S.; Eichler, J.; Ivanova, N.; Axen, S.D.; Kerfeld, C.A.; Chen, F.; Kyrpides, N.; Hugenholtz, P.; Cheng,J.-F.; Sale, K.L.; Simmons, B.; Rubin, E.
    Identification of a haloalkaliphilic and thermostable cellulase with improved ionic liquid tolerance (2011), Green Chem., 13, 2083.
No PubMed abstract available
Search for more literature for 3.2.1.91

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular about 39% of the crude enzyme activity Halorhabdus utahensis
-
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soluble about 59% of the crude enzyme activity Halorhabdus utahensis
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-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl enzyme is active in salt concentrations up to 5 M NaCl. The tolerances to heat, alkali and ionic liquids are salt-dependent. At 37°C, the optimal salt concentration is 0.25 M NaCl, at 80°C, the optimal salt concentration is 5 M NaCl Halorhabdus utahensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 x * 76000, calculated including signal peptide, x * 90000, SDS-PAGE of secreted protein Halorhabdus utahensis
90000
-
 x * 76000, calculated including signal peptide, x * 90000, SDS-PAGE of secreted protein Halorhabdus utahensis

Organic Solvent Stability

Organic Solvent Comment Organism
additional information enzyme is tolerant to high levels (20% w/w) of ionic liquids, including 1-allyl-3-methylimidazolium chloride Halorhabdus utahensis

Organism

Organism UniProt Comment Textmining
Halorhabdus utahensis C7NVN0
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Halorhabdus utahensis DSM 12940 C7NVN0
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-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the N-terminal region contains the double arginine-based signature of a TAT secretion pathway signal peptide Halorhabdus utahensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O
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 Halorhabdus utahensis 4-nitrophenol + cellobiose
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 ?
4-nitrophenyl beta-D-cellobioside + H2O
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 Halorhabdus utahensis DSM 12940 4-nitrophenol + cellobiose
-
 ?
carboxymethyl cellulose + H2O
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 Halorhabdus utahensis cellobiose + ?
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 ?
carboxymethyl cellulose + H2O
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 Halorhabdus utahensis DSM 12940 cellobiose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 76000, calculated including signal peptide, x * 90000, SDS-PAGE of secreted protein Halorhabdus utahensis

Synonyms

Synonyms Comment Organism
Huta_2387 locus name Halorhabdus utahensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 the tolerances to heat, alkali and ionic liquids are salt-dependent. At 37°C, the optimal salt concentration is 0.25 M NaCl, at 80°C, the optimal salt concentration is 5 M NaCl Halorhabdus utahensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70
-
 presence of 3 M NaCl, 225% of the activity with 2 M NaCl at 37°C Halorhabdus utahensis
80
-
 presence of 5 M NaCl, 210% of the activity with 2 M NaCl at 37°C Halorhabdus utahensis
90
-
 presence of 5 M NaCl, about 30% of the activity with 2 M NaCl at 37°C Halorhabdus utahensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 presence of 2 M NaCl Halorhabdus utahensis

pH Range

pH Minimum pH Maximum Comment Organism
4.5
-
 presence of 2 M NaCl, inactive Halorhabdus utahensis
7.5 11.5 presence of 2 M NaCl Halorhabdus utahensis

General Information

General Information Comment Organism
physiological function CBH1 is encoded in a gene cluster containing multiple putative cellulolytic enzymes Halorhabdus utahensis