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Literature summary for 3.2.1.87 extracted from

  • Majkowska-Skrobek, G.; Latka, A.; Berisio, R.; Maciejewska, B.; Squeglia, F.; Romano, M.; Lavigne, R.; Struve, C.; Drulis-Kawa, Z.
    Capsule-targeting depolymerase, derived from Klebsiella KP36 phage, as a tool for the development of anti-virulent strategy (2016), Viruses, 8, E324 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Klebsiella phage KP36

General Stability

General Stability Organism
the enzyme is susceptible to SDS denaturation and proteolytic cleavage Klebsiella phage KP36

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Klebsiella pneumoniae capsule exopolysaccharides + H2O Klebsiella phage KP36
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella phage KP36 K9L8R4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA His-Bind resin column chromatography and Superdex S200 gel filtration Klebsiella phage KP36

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Klebsiella pneumoniae capsule exopolysaccharides + H2O
-
 Klebsiella phage KP36 ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 93400, calculated from amino acid sequence Klebsiella phage KP36
trimer 3 * 94000, SDS-PAGE Klebsiella phage KP36

Synonyms

Synonyms Comment Organism
depoKP36
-
 Klebsiella phage KP36
tailspike protein
-
 Klebsiella phage KP36

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 37
-
 Klebsiella phage KP36

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 highest activity between 20 and 37°C, 95% activity at 45°C, 70% activity at 56-62°C, less than 60% activity at 70°C, completely inactive at 80°C Klebsiella phage KP36

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 65 the enzyme shows 100% and 77.6% activity after 1 h at 37°C and 45°C, respectively. The enzyme is completely inactive after 30 and 10 min at 56°C and 65°C, respectively . The melting temperature is 65°C Klebsiella phage KP36

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 7
-
 Klebsiella phage KP36

pH Range

pH Minimum pH Maximum Comment Organism
4 7 about 50% activity at pH 3.0, highest activity (about 98%) between pH 4.0 and 7.0, about 10% activity at pH 8.0, completely inactive at pH 9.0 Klebsiella phage KP36

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7 the enzyme remains active from pH 4.0-7.0 with relative activities ranging from 97.1-97.8% after a 30 min incubation. The activity diminishes substantially as the pH is lowered below 4.0 or raised above 7.0, to 54.6% and 14.0% of initial activity, respectively Klebsiella phage KP36

pI Value

Organism Comment pI Value Maximum pI Value
Klebsiella phage KP36 calculated from amino acid sequence
-
 5

General Information

General Information Comment Organism
physiological function the enzyme significantly inhibits Klebsiella-induced mortality of Galleria mellonella larvae in a time-dependent manner. The enzyme does not affect the action of antibiotics against Klebsiella pneumoniae strains Klebsiella phage KP36