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Literature summary for 3.2.1.83 extracted from

  • Yu, Y.; Liu, Z.; Yang, M.; Chen, M.; Wei, Z.; Shi, L.; Li, L.; Mou, H.
    Characterization of full-length and truncated recombinant kappa-carrageenase expressed in Pichia pastoris (2017), Front. Microbiol., 8, 1544 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.83

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 177.8% activity at 5 mM Zobellia sp. M-2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris GS-115 cells Zobellia sp. M-2

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 20.2% residual activity at 5 mM Zobellia sp. M-2
Fe2+ 68.2% residual activity at 5 mM Zobellia sp. M-2
Fe3+ 77.8% residual activity at 5 mM Zobellia sp. M-2
K+ 34.6% residual activity at 100 mM Zobellia sp. M-2
additional information EDTA, TritonX-100, Tween-80, methanol, and glycerol have minimal effects on the activity of the enzyme Zobellia sp. M-2
SDS 16.2% residual activity at 5 mM Zobellia sp. M-2
Zn2+ 28% residual activity at 5 mM Zobellia sp. M-2

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 118.5 % activity at 10 mM Zobellia sp. M-2
Mg2+ 104.3% activity at 100 mM Zobellia sp. M-2
additional information not significantly activated by Ca2+ Zobellia sp. M-2
Na+ 129.1% activity at 100 mM Zobellia sp. M-2

Organism

Organism UniProt Comment Textmining
Zobellia sp. M-2 S5U3X4
-
-

Purification (Commentary)

Purification (Comment) Organism
CM-Sepharose column chromatography Zobellia sp. M-2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageen + H2O
-
 Zobellia sp. M-2 kappa-neocarrahexaose + kappa-neocarratetraose
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Zobellia sp. M-2

Synonyms

Synonyms Comment Organism
CgkZ
-
 Zobellia sp. M-2

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant enzyme Zobellia sp. M-2

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 55 the recombinant enzyme shows the highest activity at 50°C and its relative activity decreases to 89% at 55°C Zobellia sp. M-2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42 50 after incubation at 45°C for 4 h, approximately 50% of the enzymatic activity is retained. When the recombinant enzyme is incubated at 42.5°C for 4 h, more than 90% of the enzymatic activity is retained. The recombinant enzyme is stable below 42.5°C. Approximately 50% of the enzymatic activity is lost after 1 h of incubation at 47.5°C and after 30 min of incubation at 50°C Zobellia sp. M-2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant enzyme Zobellia sp. M-2

pH Range

pH Minimum pH Maximum Comment Organism
4 8 For recombinant enzyme, at pH 4.0 or 8.0, the relative activity decreases to approximately 76% Zobellia sp. M-2