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Literature summary for 3.2.1.82 extracted from
- Purification and characterization of an exo-polygalacturonase from Pycnoporus sanguineus (2009), Mycol. Res., 113, 1404-1410.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.55 | - |
citric pectin | pH 6.8, 37°C | Trametes sanguinea | |
| 0.55 | - |
poly(1,4-alpha-D-galactosiduronate) | pH 6.8, 37°C | Trametes sanguinea |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
1 * 42000, SDS-PAGE | Trametes sanguinea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citric pectin + H2O | Trametes sanguinea | PGase I is an exocellular enzyme releasing galacturonic acid as its principal hydrolysis product, it hydrolyzes digalacturonate, and it shows a higher affinity towards poly(1,4-alpha-D-galactosiduronate) than citric pectin | ? | - |
? | |
| poly(1,4-alpha-D-galactosiduronate) + H2O | Trametes sanguinea | i.e. PGA, PGase I is an exocellular enzyme releasing galacturonic acid as its principal hydrolysis product, it hydrolyzes digalacturonate, and it shows a higher affinity towards PGA than citric pectin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes sanguinea | - |
grown on citrus fruit pectin | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | PGase I is an N-glycosidated monomer | Trametes sanguinea |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 200fold by ammonium sulfate fractionation, dialysis, and gel filtration | Trametes sanguinea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Trametes sanguinea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 500 | - |
purified native enzyme | Trametes sanguinea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citric pectin + H2O | PGase I is an exocellular enzyme releasing galacturonic acid as its principal hydrolysis product, it hydrolyzes digalacturonate, and it shows a higher affinity towards poly(1,4-alpha-D-galactosiduronate) than citric pectin | Trametes sanguinea | ? | - |
? | |
| additional information | reaction product determination and analysis, overview | Trametes sanguinea | ? | - |
? | |
| poly(1,4-alpha-D-galactosiduronate) + H2O | i.e. PGA, PGase I is an exocellular enzyme releasing galacturonic acid as its principal hydrolysis product, it hydrolyzes digalacturonate, and it shows a higher affinity towards PGA than citric pectin | Trametes sanguinea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Trametes sanguinea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-polygalacturonase | - |
Trametes sanguinea |
| PGase I | - |
Trametes sanguinea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Trametes sanguinea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | activity range | Trametes sanguinea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified native enzyme, stable up to | Trametes sanguinea |
| 70 | - |
purified native enzyme, loss of 57% activity after 30 min, pH 4.8 | Trametes sanguinea |
| 80 | - |
purified native enzyme, loss of 90% activity after 30 min, pH 4.8 | Trametes sanguinea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Trametes sanguinea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | activity range, profile, overview | Trametes sanguinea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | 8 | purified native enzyme, stable | Trametes sanguinea |
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