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Literature summary for 3.2.1.81 extracted from

  • Jang, M.K.; Lee, S.W.; Lee, D.G.; Kim, N.Y.; Yu, K.H.; Jang, H.J.; Kim, S.; Kim, A.; Lee, S.H.
    Enhancement of the thermostability of a recombinant beta-agarase, AgaB, from Zobellia galactanivorans by random mutagenesis (2010), Biotechnol. Lett., 32, 943-949.
    View publication on PubMed
Search for more literature for 3.2.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Zobellia galactanivorans

Protein Variants

Protein Variants Comment Organism
E99K the mutant shows 140% relative activity compared to the wild type enzyme Zobellia galactanivorans
E99K/T307I the mutant shows 200% relative activity compared to the wild type enzyme Zobellia galactanivorans
T307I the mutant shows 190% relative activity compared to the wild type enzyme Zobellia galactanivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the activity and stability of the wild type enzyme at 45°C with 1 mM CaCl2 are double those of the enzyme at 40°C without CaCl2. Activities of both the wild type and mutant E99K/T307I enzymes increase in 1 mM CaCl2. The E99K/T307I mutant enzyme is stable at 55°C with 1 mM CaCl2, reaching 260% of the activity the wild type enzyme held at 40°C without CaCl2. No further increases in activity are observed in 10 mMCaCl2 Zobellia galactanivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 SDS-PAGE Zobellia galactanivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agarose + H2O Zobellia galactanivorans
-
 neoagarotetraose
-
 ?
agarose + H2O Zobellia galactanivorans KCTC 12921
-
 neoagarotetraose
-
 ?

Organism

Organism UniProt Comment Textmining
Zobellia galactanivorans
-
-
-
Zobellia galactanivorans KCTC 12921
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chitin bead column chromatography Zobellia galactanivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Zobellia galactanivorans neoagarotetraose
-
 ?
agarose + H2O
-
 Zobellia galactanivorans KCTC 12921 neoagarotetraose
-
 ?

Synonyms

Synonyms Comment Organism
AgaB
-
 Zobellia galactanivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 mutant enzymes E99K, T307I and E99K/T307I are stable up to 50°C, the melting temperature of E99K/T307I is increased by 5.2°C over that of the wild type enzyme (54.6°C). The E99K/T307I mutant enzyme is stable at 55°C with 1 mM CaCl2, reaching 260% of the activity the wild type enzyme held at 40°C without CaCl2 Zobellia galactanivorans