Cloned (Comment) | Organism |
---|---|
the deduced enzyme protein contains a N-terminal signal peptide of 29 amino acid residues, followed by a 266 amino acid sequence that is homologous to catalytic module of family 16 glycoside hydrolases, a bacterial immunoglobulin group 2-like domain of 52 amino acid residues, two carbohydratebinding modules of family 6 separated from Big-2-like domain by nine times repeated GDDTDP amino acid sequence. Expression in Escherichia coli | Vibrio sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AlCl3 | complete inhibition | Vibrio sp. | |
CuCl2 | complete inhibition | Vibrio sp. | |
CuSO4 | complete inhibition | Vibrio sp. | |
HgCl2 | complete inhibition | Vibrio sp. | |
Pb(CH3COO)2 | complete inhibition | Vibrio sp. | |
sodium dodecylsulfate | 0.1%, complete inhibition | Vibrio sp. | |
ZnCl2 | complete inhibition | Vibrio sp. | |
ZnSO4 | complete inhibition | Vibrio sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for agarose is 1.1 mg/ml | Vibrio sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | slight activation | Vibrio sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106062 | - |
x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein | Vibrio sp. |
120000 | - |
x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein | Vibrio sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. | Q0KKW9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a signal peptide of 29 amino acids | Vibrio sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.4 | - |
pH 7.5, 40°C | Vibrio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agar + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? | |
agarose + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? | |
additional information | no substrate: neoagarotetraose, neoagarobiose, kappa-carrageenan | Vibrio sp. | ? | - |
? | |
neoagarohexaose + H2O | poor substrate | Vibrio sp. | ? | - |
? | |
neoagarooctaose + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose + neoagarobiose | - |
? | |
porphyran + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein | Vibrio sp. |
Synonyms | Comment | Organism |
---|---|---|
beta-agarase-a | - |
Vibrio sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Vibrio sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable below | Vibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Vibrio sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 11 | stable at 25°C for 24 h | Vibrio sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Vibrio sp. | calculated | - |
4.1 |