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Literature summary for 3.2.1.81 extracted from

  • Bibb, M.J.; Jone, G.H.; Joseph, R.; Buttner, M.J.; Ward, J.M.
    The agarase gene (dagA) of Streptomyces coelicolor A3(2): affinity purification and characterization of the cloned gene product (1987), J. Gen. Microbiol., 133, 2089-2096.
    View publication on PubMed
Search for more literature for 3.2.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces lividans, natural Dag- Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ in higher concentrations, 10-20 mM Streptomyces coelicolor
Mn2+ in higher concentrations, 10-20 mM Streptomyces coelicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Streptomyces coelicolor 5622
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agarose + H2O Streptomyces coelicolor
-
 neoagarotetraose
-
 ir

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification
-
 Streptomyces coelicolor

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces coelicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
616
-
-
 Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Streptomyces coelicolor neoagarotetraose
-
 ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 in imidazol or acetate buffers Streptomyces coelicolor
7.5
-
 in Tris buffer, activity significant lower Streptomyces coelicolor

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 in imidazol or acetate buffers Streptomyces coelicolor