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Literature summary for 3.2.1.8 extracted from
- Preparation of a robust immobilized biocatalyst of beta-1,4-endoxylanase by surface coating with polymers for production of xylooligosaccharides from different xylan sources (2018), New Biotechnol., 44, 50-58 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene xysA, recombinant expression of C-terminally His-tagged Xys1 in Streptomyces lividans, the enzyme is secreted | Streptomyces halstedii |
| overexpressed in Streptomyces lividans | Streptomyces halstedii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | preparation of a robust immobilized biocatalyst of beta-1,4-endoxylanase C-terminally His-tagged Xys1 by surface coating with polymers for production of xylooligosaccharides from different xylan sources. Surface coating of dextran-modified Ag-G-Xys1-L and Ag-G-Xys1-H biocatalysts with cationic polymer (PEI) by chemical modifications via ionic exchange. The optimized biocatalyst is 550fold more stable than one-point covalent immobilized C-terminally His-tagged Xys1 at 70°C, pH 7.0. Hydrolysis of beechwood, wheat straw and corncob xylans is 93% in 4 h, 44% in 5 h and 100% in 1 h, respectively. Maximum values of xylooligosaccharides are found for beechwood at 20.6 mg/ml, wheat at 12.5 mg/ml, and corncob at 30.4 mg/ml. The optimized biocatalyst is reused for 15 reaction cycles without affecting its catalytic activity. Method optmization and evaluation, overview | Streptomyces halstedii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces halstedii | - |
- |
- |
| Streptomyces halstedii | Q59922 | - |
- |
| Streptomyces halstedii JM8 | - |
- |
- |
| Streptomyces halstedii JM8 | Q59922 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant extracellular C-terminally His-tagged Xys1 from Streptomyces lividans | Streptomyces halstedii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beechwood xylan + H2O | - |
Streptomyces halstedii | ? | - |
? |  |
| beechwood xylan + H2O | hydolysis is 93% in 4 h. Maximum values of xylooligosaccharides are found for beechwood at 20.6 mg/ml. Enzyme immobilized and stabilized on glyoxyl-agarose beads by multipoint covalent attachment, optimal modification consists of surface coating with a bilayer formed by a layer of derived dextran polymers and a layer of polyethylenimine | Streptomyces halstedii | ? | - |
? | |
| beechwood xylan + H2O | hydolysis is 93% in 4 h. Maximum values of xylooligosaccharides are found for beechwood at 20.6 mg/ml. Enzyme immobilized and stabilized on glyoxyl-agarose beads by multipoint covalent attachment, optimal modification consists of surface coating with a bilayer formed by a layer of derived dextran polymers and a layer of polyethylenimine | Streptomyces halstedii JM8 | ? | - |
? | |
| beechwood xylan + H2O | - |
Streptomyces halstedii JM8 | ? | - |
? | |
| corncob xylan + H2O | - |
Streptomyces halstedii | ? | - |
? | |
| corncob xylan + H2O | hydolysis is 100% in 1 h. Maximum values of xylooligosaccharides are found for beechwood at 30.4 mg/ml. Enzyme immobilized and stabilized on glyoxyl-agarose beads by multipoint covalent attachment, optimal modification consists of surface coating with a bilayer formed by a layer of derived dextran polymers and a layer of polyethylenimine | Streptomyces halstedii | ? | - |
? | |
| corncob xylan + H2O | hydolysis is 100% in 1 h. Maximum values of xylooligosaccharides are found for beechwood at 30.4 mg/ml. Enzyme immobilized and stabilized on glyoxyl-agarose beads by multipoint covalent attachment, optimal modification consists of surface coating with a bilayer formed by a layer of derived dextran polymers and a layer of polyethylenimine | Streptomyces halstedii JM8 | ? | - |
? | |
| corncob xylan + H2O | - |
Streptomyces halstedii JM8 | ? | - |
? | |
| wheat straw + H2O | hydolysis is 44% in 5 h. Maximum values of xylooligosaccharides are found for beechwood at 12.5 mg/ml. Enzyme immobilized and stabilized on glyoxyl-agarose beads by multipoint covalent attachment, optimal modification consists of surface coating with a bilayer formed by a layer of derived dextran polymers and a layer of polyethylenimine | Streptomyces halstedii | ? | - |
? | |
| wheat straw + H2O | hydolysis is 44% in 5 h. Maximum values of xylooligosaccharides are found for beechwood at 12.5 mg/ml. Enzyme immobilized and stabilized on glyoxyl-agarose beads by multipoint covalent attachment, optimal modification consists of surface coating with a bilayer formed by a layer of derived dextran polymers and a layer of polyethylenimine | Streptomyces halstedii JM8 | ? | - |
? | |
| wheat straw xylan + H2O | - |
Streptomyces halstedii | ? | - |
? | |
| wheat straw xylan + H2O | - |
Streptomyces halstedii JM8 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,4-endoxylanase | - |
Streptomyces halstedii |
| endoxylanase Xys1DELTA | - |
Streptomyces halstedii |
| xysA | - |
Streptomyces halstedii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Streptomyces halstedii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptomyces halstedii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme is immobilized and stabilized on glyoxyl-agarose beads by multipoint covalent attachment using a novel strategy based on surface coating with a multilayer of polymers. The optimal modification consists of surface coating with a bilayer formed by a layer of derived dextran polymers and a layer of polyethylenimine | Streptomyces halstedii |
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