Literature summary for 3.2.1.8 extracted from

Rashamuse, K.; Sanyika Tendai, W.; Mathiba, K.; Ngcobo, T.; Mtimka, S.; Brady, D.
Metagenomic mining of glycoside hydrolases from the hindgut bacterial symbionts of a termite (Trinervitermes trinervoides) and the characterization of a multimodular beta-1,4-xylanase (GH11) (2017), Biotechnol. Appl. Biochem., 64, 174-186 .

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Protein Variants

Protein Variants	Comment	Organism
additional information	construction of truncated mutants, lacking the carbohydrate binding module CBM, which functions to selectively bind insoluble xylan and increase the rate of hydrolysis. Xyl1A corresponds to the GH11 domain coding sequence, Xyl1B corresponds to the GH11 and the CBM1 domain coding sequences, and Xyl1C corresponds to the two CBM coding sequences	uncultured bacterium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	beechwood xylan	Km value 1.2 mg/ml for wild-type, 2.8 mg/ml for mutant XylA, 1.9 mg/ml for mutant XylB, pH 6.0, 50°C	uncultured bacterium

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	A0A127JBT0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beechwood xylan + H2O	-	uncultured bacterium	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 55000, wild-type, x * 28000, mutant XylA, x * 40000, mutant XylB, x * 26000, mutant XylC, SDS-PAGE	uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	uncultured bacterium

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	60	more than 60% of maximum activity	uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
38.9	-	beechwood xylan	mutant XylA, pH 6.0, 50°C	uncultured bacterium
49.6	-	beechwood xylan	mutant XylB, pH 6.0, 50°C	uncultured bacterium
55.3	-	beechwood xylan	wild-type, pH 6.0, 50°C	uncultured bacterium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	uncultured bacterium

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	more than 80% of maximum activity	uncultured bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
additional information	-	beechwood xylan	kcat/Km value 47 ml/mg/s for wild-type, 13.9 ml/mg/s for mutant XylA, 26.1 ml/mg/s for mutant XylB, pH 6.0, 50°C	uncultured bacterium

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Release 2023.1 (January 2023)