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Literature summary for 3.2.1.8 extracted from

  • Jun, H.; Bing, Y.; Keying, Z.; Xuemei, D.; Daiwen, C.
    Sequencing and expression of the xylanase gene 2 from Trichoderma reesei Rut C-30 and characterization of the recombinant enzyme and its activity on xylan (2009), J. Mol. Microbiol. Biotechnol., 17, 101-109.
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
gene xyn2, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme without N-terminal signal in Escherichia coli Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for beta-1,4-xylan from birchwood is 0.15 mg/ml Trichoderma reesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 x * 21000, recombinant His-tagged mature enzyme without N-terminal signal sequence, SDS-PAGE Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Trichoderma reesei
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei B2CNY5 Xyn2 or Rut C-30; gene xyn2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged Xyn2 1.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Trichoderma reesei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2350
-
 purified recombinant enzyme Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
 Trichoderma reesei ?
-
 ?
beta-1,4-xylan + H2O from birchwood Trichoderma reesei ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, recombinant His-tagged mature enzyme without N-terminal signal sequence, SDS-PAGE Trichoderma reesei

Synonyms

Synonyms Comment Organism
endo-beta-1,4-xylanase2
-
 Trichoderma reesei
Rut C-30
-
 Trichoderma reesei
xylanase
-
 Trichoderma reesei
Xyn2
-
 Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Trichoderma reesei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30% of maximal activity at 70°C Trichoderma reesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 over 85% activity remaining after 30 min Trichoderma reesei
60
-
 over 65% activity remaining after 30 min Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
199.7
-
 beta-1,4-xylan pH 5.0, 50°C, birchwood xylan Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Trichoderma reesei

pH Range

pH Minimum pH Maximum Comment Organism
3 8 activity range, 40% of maximal activity at pH 3.0 and pH 8.0 Trichoderma reesei

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 7.5 purified recombinant enzyme, stable Trichoderma reesei

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma reesei isoelectric focusing
-
 7.5