Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates 16% at 10 mM | Bispora sp. MEY-1 |
Cloned (Comment) | Organism |
---|---|
gene xyl10C from genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Pichia pastoris | Bispora sp. MEY-1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bispora sp. MEY-1 | |
Cu2+ | - |
Bispora sp. MEY-1 | |
Fe3+ | - |
Bispora sp. MEY-1 | |
Mg2+ | - |
Bispora sp. MEY-1 | |
additional information | poor inhibition by Ni2+ and Co2+ | Bispora sp. MEY-1 | |
Pb2+ | - |
Bispora sp. MEY-1 | |
SDS | complete inhibition at 5 mM | Bispora sp. MEY-1 | |
Zn2+ | - |
Bispora sp. MEY-1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | activates 66.5% at 1 mM | Bispora sp. MEY-1 | |
Cr3+ | activates 19% at 10 mM | Bispora sp. MEY-1 | |
additional information | no effect by EDTA, Li+, Na+, and K+ | Bispora sp. MEY-1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 449000, Xyl10C, sequence calculation, x * 90000, about, glycosylated recombinant Xyl10C, SDS-PAGE, x * 55000, deglycosylated recombinant Xyl10C, SDS-PAGE | Bispora sp. MEY-1 |
90000 | - |
x * 449000, Xyl10C, sequence calculation, x * 90000, about, glycosylated recombinant Xyl10C, SDS-PAGE, x * 55000, deglycosylated recombinant Xyl10C, SDS-PAGE | Bispora sp. MEY-1 |
449000 | - |
x * 449000, Xyl10C, sequence calculation, x * 90000, about, glycosylated recombinant Xyl10C, SDS-PAGE, x * 55000, deglycosylated recombinant Xyl10C, SDS-PAGE | Bispora sp. MEY-1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Bispora sp. MEY-1 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bispora sp. MEY-1 | D0QF43 | CGMCC 2500, gene xyl10C | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Bispora sp. MEY-1 |
Purification (Comment) | Organism |
---|---|
recombinant Xyl10C 1.1fold from Pichia pastoris by anion exchange chromatography | Bispora sp. MEY-1 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | grown on wheat bran | Bispora sp. MEY-1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18830 | - |
purified recombinant Xyl10C | Bispora sp. MEY-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | - |
Bispora sp. MEY-1 | ? | - |
? | |
beta-1,4-xylan + H2O | from oat spelt | Bispora sp. MEY-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 449000, Xyl10C, sequence calculation, x * 90000, about, glycosylated recombinant Xyl10C, SDS-PAGE, x * 55000, deglycosylated recombinant Xyl10C, SDS-PAGE | Bispora sp. MEY-1 |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-xylanase | - |
Bispora sp. MEY-1 |
More | the enzyme belongs to the glycosyl hydrolase family 10, GH10 | Bispora sp. MEY-1 |
XYL10C | - |
Bispora sp. MEY-1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
recombinant Xyl10C | Bispora sp. MEY-1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 100 | activity range, 80% of maximal activity at 75-90°C, 20% at 100°C, and 10% at 40°C, profile, overview | Bispora sp. MEY-1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
purified recombinant Xyl10C, 60 min, completely stablem half-life is 45 h | Bispora sp. MEY-1 |
85 | - |
purified recombinant Xyl10C, half-life is 3 h | Bispora sp. MEY-1 |
90 | - |
purified recombinant Xyl10C, 10 min, 87% remaining activity | Bispora sp. MEY-1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | recombinant Xyl10C | Bispora sp. MEY-1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 7 | first peak at pH 3.0 with 63% of maximal activity, and second peak with maximal activity at pH 4.5-5.0, profile, overview | Bispora sp. MEY-1 |