Cloned (Comment) | Organism |
---|---|
gene xynA19, phylogenetic tree, expression of His-tagged XynA19 without the signal peptide sequence in Escherichia coli strains BL21 (DE3) and Top10 | Sphingobacterium sp. TN19 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for the recombinant enzyme is 17.9 mg/ml | Sphingobacterium sp. TN19 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Sphingobacterium sp. TN19 | - |
D-xylose + beta-1,4-xylobiose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobacterium sp. TN19 | D8L2Y2 | a symbiotic bacterium isolated from the gut of Batocera horsfieldi larvae, gene xynA19 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged XynA19 from Escherichia coli strains Top10 and BL21(DE3) by ultrafiltration and nickel affinity chromatography | Sphingobacterium sp. TN19 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
196.9 | - |
purified recombinant enzyme | Sphingobacterium sp. TN19 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | - |
Sphingobacterium sp. TN19 | D-xylose + beta-1,4-xylobiose + ? | - |
? | |
beta-1,4-xylan + H2O | - |
Sphingobacterium sp. TN19 | D-xylose + beta-1,4-xylobiose + ? | hydrolysis products comprise 20.1% xylose and 79.9% xylobiose | ? |
Subunits | Comment | Organism |
---|---|---|
More | XynA19 contains a complete GH10 catalytic domain of GH 10 from Ile38 to Ala381, structure homology modeling of the recombinant enzyme, overview | Sphingobacterium sp. TN19 |
Synonyms | Comment | Organism |
---|---|---|
More | XynA19 belongs to glycosyl hydrolase family 10, GH 10 | Sphingobacterium sp. TN19 |
xylanase | - |
Sphingobacterium sp. TN19 |
XynA19 | - |
Sphingobacterium sp. TN19 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Sphingobacterium sp. TN19 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 65 | the enzyme shows over 65% of its maximum activity at 20-28°C and about 40% at 10°C | Sphingobacterium sp. TN19 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
purified recombinant enzyme, pH 6.5, 40% remaining activity | Sphingobacterium sp. TN19 |
20 | - |
purified recombinant enzyme, pH 6.5, 65% remaining activity | Sphingobacterium sp. TN19 |
30 | 50 | purified recombinant enzyme, pH 6.5, over 80% remaining activity, above 50°C the enzyme activity decreases rapidly after 10 min preincubation | Sphingobacterium sp. TN19 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
603.8 | - |
beta-1,4-xylan | pH 6.5, 45°C | Sphingobacterium sp. TN19 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Sphingobacterium sp. TN19 |