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Literature summary for 3.2.1.8 extracted from

  • Yoon, K.H.; Yun, H.N.; Jung, K.H.
    Molecular cloning of a Bacillus sp. KK-1 xylanase gene and characterization of the product (1998), Biochem. Mol. Biol. Int., 45, 337-347.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44700
-
x * 44700, recombinat enzyme, calculation from nucleotide sequence Bacillus sp. (in: Bacteria)
45000
-
x * 45000, recombinant enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
enzyme XYLY
-
Bacillus sp. (in: Bacteria) KK-1
-
enzyme XYLY
-

Purification (Commentary)

Purification (Comment) Organism
enzyme XYLY, from recombinant Escherichia coli Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O birchwood xylan Bacillus sp. (in: Bacteria) additional information
-
?
1,4-beta-D-xylan + H2O oat spelt xylan Bacillus sp. (in: Bacteria) additional information
-
?
1,4-beta-D-xylan + H2O birchwood xylan Bacillus sp. (in: Bacteria) KK-1 additional information
-
?
beta-glucan + H2O barley beta-glucan Bacillus sp. (in: Bacteria) ?
-
?
beta-glucan + H2O barley beta-glucan Bacillus sp. (in: Bacteria) KK-1 ?
-
?
carboxymethylcellulose + H2O no activity Bacillus sp. (in: Bacteria) ?
-
?
laminarin + H2O no activity Bacillus sp. (in: Bacteria) ?
-
?
laminarin + H2O no activity Bacillus sp. (in: Bacteria) KK-1 ?
-
?
lichenan + H2O low activity Bacillus sp. (in: Bacteria) ?
-
?
lichenan + H2O low activity Bacillus sp. (in: Bacteria) KK-1 ?
-
?
starch + H2O no activity Bacillus sp. (in: Bacteria) ?
-
?
starch + H2O no activity Bacillus sp. (in: Bacteria) KK-1 ?
-
?

Subunits

Subunits Comment Organism
? x * 45000, recombinant enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)
? x * 44700, recombinat enzyme, calculation from nucleotide sequence Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
recombinant enzyme from Bacillus sp. (in: Bacteria)
70
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
30 min, 70% loss of activity, recombinant enzyme Bacillus sp. (in: Bacteria)