Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Bacillus sp. SWU60 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 96% residual activity at 1 mM | Bacillus sp. SWU60 | |
EDTA | 78% residual activity at 1 mM | Bacillus sp. SWU60 | |
Hg2+ | 4% residual activity at 1 mM | Bacillus sp. SWU60 | |
Mn2+ | 67% residual activity at 1 mM | Bacillus sp. SWU60 | |
Na+ | 87% residual activity at 1 mM | Bacillus sp. SWU60 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 106% activity at 1 mM | Bacillus sp. SWU60 | |
Cd2+ | 104% activity at 1 mM | Bacillus sp. SWU60 | |
Cu2+ | 106% activity at 1 mM | Bacillus sp. SWU60 | |
additional information | not influenced by Co2+ and Ni2+ | Bacillus sp. SWU60 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. SWU60 | A0A0K2S4R5 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Bacillus sp. SWU60 |
Purification (Comment) | Organism |
---|---|
butyl-Toyopearl 650 M column chromatography, DEAE-Toyopearl 650 M column chromatography, Mono Q column chromatography, Mono S column chromatography, Superdex 75 gel filtration, and HisTrap column chromatography | Bacillus sp. SWU60 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
crude enzyme, at pH 6.0 and 60°C | Bacillus sp. SWU60 |
14 | - |
after 46.7fold purification, at pH 6.0 and 60°C | Bacillus sp. SWU60 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-mannan + H2O | 87% activity compared to konjac glucomannan | Bacillus sp. SWU60 | mannose + mannobiose + mannotriose | - |
? | |
carob galactomannan + H2O | 56% activity compared to konjac glucomannan | Bacillus sp. SWU60 | ? | - |
? | |
konjac glucomannan + H2O | 100% activity | Bacillus sp. SWU60 | ? | - |
? | |
locust bean gum + H2O | - |
Bacillus sp. SWU60 | ? | - |
? | |
additional information | the enzyme does not show activity toward guar galactomannan, 4-nitrophenyl beta-D-mannopyranoside, mannobiose, birchwood xylan, or carboxymethyl cellulose | Bacillus sp. SWU60 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, native purified enzyme, SDS-PAGE | Bacillus sp. SWU60 |
? | x * 54000, recombinant protein, SDS-PAGE | Bacillus sp. SWU60 |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-mannanase | - |
Bacillus sp. SWU60 |
ManS2 | - |
Bacillus sp. SWU60 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. SWU60 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | more than 60% activity between 30 and 80°C | Bacillus sp. SWU60 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme is stable up to 60°C for 1 h. At 70°C, the activity drops to less than 40% after 1h | Bacillus sp. SWU60 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus sp. SWU60 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | more than 60% activity between pH 5.0 and 8.0 | Bacillus sp. SWU60 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | the enzyme is stable at pH 5.0-9.0 at 4 °C for 16 h | Bacillus sp. SWU60 |