Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | about 110% activity at 100 mM | insect gut metagenome | |
dithiothreitol | about 105% activity at 100 mM | insect gut metagenome |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | insect gut metagenome |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | about 55% residual activity at 10% (w/v) | insect gut metagenome | |
Benzene | complete inhibition at 10% (w/v) | insect gut metagenome | |
chloroform | complete inhibition at 10% (w/v) | insect gut metagenome | |
Co2+ | about 90% residual activity at 1 mM | insect gut metagenome | |
CTAB | complete inhibition at 10% (v/v) | insect gut metagenome | |
Cyclohexane | about 5% residual activity at 10% (w/v) | insect gut metagenome | |
Fe2+ | about 35% residual activity at 1 mM | insect gut metagenome | |
Hg2+ | complete inhibition at 1 mM | insect gut metagenome | |
Mn2+ | about 93% residual activity at 1 mM | insect gut metagenome | |
additional information | EDTA has no effect on enzyme activity | insect gut metagenome | |
n-butanol | complete inhibition at 10% (w/v) | insect gut metagenome | |
n-Heptanol | about 5% residual activity at 10% (w/v) | insect gut metagenome | |
n-hexane | about 5% residual activity at 10% (w/v) | insect gut metagenome | |
n-Hexanol | about 5% residual activity at 10% (w/v) | insect gut metagenome | |
n-Pentanol | about 10% residual activity at 10% (w/v) | insect gut metagenome | |
SDS | complete inhibition at 10% (w/v) | insect gut metagenome | |
Toluene | about 5% residual activity at 10% (w/v) | insect gut metagenome | |
Triton X-100 | about 55% residual activity at 10% (v/v) | insect gut metagenome | |
Tween 20 | about 60% residual activity at 10% (v/v) | insect gut metagenome | |
Tween 40 | about 58% residual activity at 10% (v/v) | insect gut metagenome | |
Tween 80 | about 61% residual activity at 10% (v/v) | insect gut metagenome | |
Urea | about 70% residual activity at 100 mM | insect gut metagenome |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by Mg2+, Ca2+, Ni2+, and Cd2+ | insect gut metagenome | |
Zn2+ | about 105% activity at 1 mM | insect gut metagenome |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | the enzyme activity remains stable in the presence of 10% (v/v) | insect gut metagenome |
dimethyl formamide | the enzyme activity remains stable in the presence of 10% (v/v) | insect gut metagenome |
dimethyl sulfoxide | the enzyme activity remains stable in the presence of 10% (v/v) | insect gut metagenome |
Ethanol | the enzyme activity remains stable in the presence of 10% (v/v) | insect gut metagenome |
Methanol | the enzyme activity remains stable in the presence of 10% (v/v) | insect gut metagenome |
propanol | the enzyme activity remains stable in the presence of 10% (v/v) | insect gut metagenome |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
insect gut metagenome | A0A1W6DZL1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
insect gut metagenome |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | insect gut metagenome |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-mannan + H2O | - |
insect gut metagenome | ? | - |
? | |
guar gum galactomannan + H2O | - |
insect gut metagenome | ? | - |
? | |
konjac glucomannan + H2O | - |
insect gut metagenome | ? | - |
? | |
locust bean gum galactomannan + H2O | highest activity | insect gut metagenome | ? | - |
? | |
additional information | no activity with 4-nitrophenyl beta-D-mannopyranoside | insect gut metagenome | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | insect gut metagenome |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-mannanase | - |
insect gut metagenome |
ManEM17 | - |
insect gut metagenome |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
insect gut metagenome |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | more than 60% activity between 45 and 60°C | insect gut metagenome |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | below 45°C, the recombinant enzyme remains stable with 95% glycosyl hydrolase activity for 1 h, but quickly loses this function above 55°C | insect gut metagenome |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
insect gut metagenome |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | long-term (15 h) pre-exposure to acidic and alkaline conditions (pH 5.0-9.5 at 4°C) does not significantly affect enzymatic function, and at least 65% residual activity is retained | insect gut metagenome |