Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.75 extracted from

  • Temple, M.J.; Cuskin, F.; Basle, A.; Hickey, N.; Speciale, G.; Williams, S.J.; Gilbert, H.J.; Lowe, E.C.
    A Bacteroidetes locus dedicated to fungal 1,6-beta-glucan degradation Unique substrate conformation drives specificity of the key endo-1,6-beta-glucanase (2017), J. Biol. Chem., 292, 10639-10650 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner cells Bacteroides thetaiotaomicron

Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme or in complex with beta-glucosyl-1,6-deoxynojirimycin, hanging drop vapor diffusion method, using 0.2 M sodium bromide, 0.1 M Bis-Tris propane, pH 6.5, 20% w/v PEG 3350 or 0.2 M potassium thiocyanate 20% w/v PEG 3350, respectively Bacteroides thetaiotaomicron

Protein Variants

Protein Variants Comment Organism
C393S the mutant shows 0.005% activity compared to the wild type enzyme Bacteroides thetaiotaomicron
C396S the mutant shows 0.03% activity compared to the wild type enzyme Bacteroides thetaiotaomicron
D286A the mutant shows 0.27% activity compared to the wild type enzyme Bacteroides thetaiotaomicron
E328A inactive Bacteroides thetaiotaomicron
E339A inactive Bacteroides thetaiotaomicron
E339Q inactive Bacteroides thetaiotaomicron
H281A the mutant shows 0.006% activity compared to the wild type enzyme Bacteroides thetaiotaomicron
N282A the mutant shows 0.009% activity compared to the wild type enzyme Bacteroides thetaiotaomicron
W345A the mutant shows 0.004% activity compared to the wild type enzyme Bacteroides thetaiotaomicron

Inhibitors

Inhibitors Comment Organism Structure
beta-glucosyl-1,6-deoxynojirimycin
-
 Bacteroides thetaiotaomicron

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Bacteroides thetaiotaomicron 9986
-

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron Q8A2J3
-
-
Bacteroides thetaiotaomicron VPI-5482 Q8A2J3
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon Co2+ affinity column chromatography and Superdex 200 gel filtration Bacteroides thetaiotaomicron

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:1,6-beta-glucan-grown cell
-
 Bacteroides thetaiotaomicron
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,6-beta-glucohexaose + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
1,6-beta-glucohexaose + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?
1,6-beta-glucooctaose + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
1,6-beta-glucooctaose + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?
1,6-beta-glucotetraose + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
1,6-beta-glucotetraose + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?
1,6-beta-glucotriose + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
additional information the enzyme shows no activity on gentiobiose, 1,3-beta-glucan, 1,4-beta-glucan, or 1,6-beta-galactan Bacteroides thetaiotaomicron ?
-
 ?
additional information the enzyme shows no activity on gentiobiose, 1,3-beta-glucan, 1,4-beta-glucan, or 1,6-beta-galactan Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?
pustulan + H2O highly specific substrate Bacteroides thetaiotaomicron gentobiose + D-glucose
-
 ?
pustulan + H2O highly specific substrate Bacteroides thetaiotaomicron VPI-5482 gentobiose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
BT3312
-
 Bacteroides thetaiotaomicron
endo-1,6-beta-glucanase
-
 Bacteroides thetaiotaomicron
PUL1,6-beta-glucan locus name Bacteroides thetaiotaomicron

Expression

Organism Comment Expression
Bacteroides thetaiotaomicron expression is up-regulated by 1,6-beta-glucan up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
28.33
-
 1,6-beta-glucotriose at pH 7.0 and 37°C Bacteroides thetaiotaomicron
43.33
-
 1,6-beta-glucotetraose at pH 7.0 and 37°C Bacteroides thetaiotaomicron
100
-
 1,6-beta-glucohexaose at pH 7.0 and 37°C Bacteroides thetaiotaomicron
106.67
-
 1,6-beta-glucooctaose at pH 7.0 and 37°C Bacteroides thetaiotaomicron