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Literature summary for 3.2.1.75 extracted from

  • Wang, D.; Kim, D.H.; Yun, E.J.; Park, Y.C.; Seo, J.H.; Kim, K.H.
    The first bacterial beta-1,6-endoglucanase from Saccharophagus degradans 2-40T for the hydrolysis of pustulan and laminarin (2017), Appl. Microbiol. Biotechnol., 101, 197-204 .
    View publication on PubMed
Search for more literature for 3.2.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharophagus degradans

Protein Variants

Protein Variants Comment Organism
food industry the enzyme can be used to hydrolyze beta-1,6-glucans of brown algae or fungal cell walls for producing gentiobiose as a high-value sugar and glucose as a fermentable sugar Saccharophagus degradans

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 70.9% residual activity at 1 mM Saccharophagus degradans
Cu2+ 29.4% residual activity at 1 mM Saccharophagus degradans
Fe2+ 61.5% residual activity at 1 mM Saccharophagus degradans
Mg2+ 57% residual activity at 1 mM Saccharophagus degradans
Mn2+ 85% residual activity at 1 mM Saccharophagus degradans
Ni2+ 54.7% residual activity at 1 mM Saccharophagus degradans

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by K+, Ca2+, or Na+ Saccharophagus degradans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
laminarin + H2O Saccharophagus degradans
-
 gentobiose + D-glucose
-
 ?
pustulan + H2O Saccharophagus degradans
-
 gentobiose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharophagus degradans Q21GC8
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Saccharophagus degradans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O
-
 Saccharophagus degradans gentobiose + D-glucose
-
 ?
laminarin + H2O 22.4% activity compared to pustulan Saccharophagus degradans gentobiose + D-glucose
-
 ?
additional information no activity with curdlan, barley beta-glucan, xylan and carboxymethyl cellulose Saccharophagus degradans ?
-
 ?
pustulan + H2O
-
 Saccharophagus degradans gentobiose + D-glucose
-
 ?
pustulan + H2O 100% activity Saccharophagus degradans gentobiose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Saccharophagus degradans
? x * 52800, calculated from amino acid sequence Saccharophagus degradans

Synonyms

Synonyms Comment Organism
beta-1,6-endoglucanase
-
 Saccharophagus degradans
gly30B
-
 Saccharophagus degradans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Saccharophagus degradans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 more than 50% activity between 20 and 60°C Saccharophagus degradans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 the enzyme remains stable from 20-40°C for 30 min. At 50°C and above, the enzyme activity drops to 20% after 30 min incubation Saccharophagus degradans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
28.9
-
 Laminarin at pH 7.0 and 40°C Saccharophagus degradans
135.6
-
 pustulan at pH 7.0 and 40°C Saccharophagus degradans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Saccharophagus degradans

pH Range

pH Minimum pH Maximum Comment Organism
4 9 more than 60% activity between pH 4.0 and 9.0 Saccharophagus degradans