Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | BGN16.3 is induced rapidly and strongly by fungal cell walls | Trichoderma harzianum |
Cloned (Comment) | Organism |
---|---|
expression profile of BGN16.3 | Trichoderma harzianum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion of the beta-1,6-glucanase multienzyme complex, BGN16.3 is part of the complex | Trichoderma harzianum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | 30000 | gel filtration | Trichoderma harzianum |
46000 | - |
? * 46000, BGN16.3, SDS-PAGE | Trichoderma harzianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trichoderma harzianum | BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview | ? | - |
? | |
additional information | Trichoderma harzianum CECT 2413 | BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma harzianum | - |
3 isozymes BGN16.1, BGN16.2, and BGN16.3 | - |
Trichoderma harzianum CECT 2413 | - |
3 isozymes BGN16.1, BGN16.2, and BGN16.3 | - |
Purification (Comment) | Organism |
---|---|
native extracellular BGN16.3 26fold to homogeneity by beta-1,6-glycan affinity chromatography, chromatofocusing, and gel filtration | Trichoderma harzianum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | expression profile of BGN16.3, regulation of BGN16.3 expression | Trichoderma harzianum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
188 | - |
purified enzyme | Trichoderma harzianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucan + H2O | substrate from yeast, 18% of maximal activity | Trichoderma harzianum | ? | - |
? | |
glucan + H2O | substrate from yeast, 18% of maximal activity | Trichoderma harzianum CECT 2413 | ? | - |
? | |
laminarin + H2O | 8% of maximal activity | Trichoderma harzianum | ? | - |
? | |
laminarin + H2O | 8% of maximal activity | Trichoderma harzianum CECT 2413 | ? | - |
? | |
additional information | BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview | Trichoderma harzianum | ? | - |
? | |
additional information | substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose | Trichoderma harzianum | ? | - |
? | |
additional information | BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview | Trichoderma harzianum CECT 2413 | ? | - |
? | |
additional information | substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose | Trichoderma harzianum CECT 2413 | ? | - |
? | |
pustulan + H2O | best substrate | Trichoderma harzianum | oligomers of pustulan | product analysis, most abundant products are di-, tri-, and tetra-beta-1,6-glucosides | ? | |
pustulan + H2O | best substrate | Trichoderma harzianum CECT 2413 | oligomers of pustulan | product analysis, most abundant products are di-, tri-, and tetra-beta-1,6-glucosides | ? |
Subunits | Comment | Organism |
---|---|---|
monomer or dimer | ? * 46000, BGN16.3, SDS-PAGE | Trichoderma harzianum |
More | BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex | Trichoderma harzianum |
Synonyms | Comment | Organism |
---|---|---|
acidic beta-1,6-glucanase | - |
Trichoderma harzianum |
BGN16.3 | - |
Trichoderma harzianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Trichoderma harzianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Trichoderma harzianum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | 20% of maximal activity at pH 4.0 and pH 7.0 | Trichoderma harzianum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trichoderma harzianum | acidic chromatofocusing | - |
4.1 |
Trichoderma harzianum | isoelectric focusing | - |
4.5 |