Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
uncultured microorganism |
Cloned (Comment) | Organism |
---|---|
gene mt-lic, genomic library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree and sequence comparisons, recombinant expression in Escherichia coli strain C43(DE3) | uncultured microorganism |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
uncultured microorganism | |
additional information | poor inhibition by Fe3+ | uncultured microorganism | |
Zn2+ | - |
uncultured microorganism |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.45 mg/ml with lichenan at pH 6.0, 50°C | uncultured microorganism |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme contains a signal peptide | uncultured microorganism | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates slightly | uncultured microorganism | |
Fe2+ | activates | uncultured microorganism | |
Mg2+ | activates | uncultured microorganism | |
Mn2+ | activates slightly | uncultured microorganism |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41694 | - |
x * 45133, sequence calculation, x * 41694, recombinant enzyme without signal peptide, SDS-PAGE | uncultured microorganism |
45133 | - |
x * 45133, sequence calculation, x * 41694, recombinant enzyme without signal peptide, SDS-PAGE | uncultured microorganism |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley-beta-glucan + H2O | uncultured microorganism | - |
? | - |
? | |
lichenan + H2O | uncultured microorganism | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured microorganism | KM079629 | isolated from soil metagenome, gene mt-lic | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain C43(DE3), the signal peptide is cleaved | uncultured microorganism |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.83 | - |
substrate lichenan, pH 6.0, 50°C | uncultured microorganism |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley-beta-glucan + H2O | - |
uncultured microorganism | ? | - |
? | |
barley-beta-glucan + H2O | best substrate, azo-barley-glucan as the substrate | uncultured microorganism | ? | - |
? | |
CM-cellulose + H2O | low activity | uncultured microorganism | ? | - |
? | |
lichenan + H2O | - |
uncultured microorganism | ? | - |
? | |
soluble starch + H2O | low activity | uncultured microorganism | ? | - |
? | |
xylan + H2O | low activity | uncultured microorganism | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45133, sequence calculation, x * 41694, recombinant enzyme without signal peptide, SDS-PAGE | uncultured microorganism |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,3-1,4-glucanase | - |
uncultured microorganism |
Lichenase | - |
uncultured microorganism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
uncultured microorganism |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | activity range, profile overview | uncultured microorganism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
uncultured microorganism |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | activity range, no activity below or above | uncultured microorganism |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 8 containing the amino acid sequence ATDGDLDIAYALLLASLQW | uncultured microorganism |