Cloned (Comment) | Organism |
---|---|
gene RmLic16A, DNA and amino acid sequence determination and analysis, sequence comparisons | Rhizomucor miehei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 2.0 mg/ml with barley beta-glucan and 1.4 mg/ml with lichenan at pH 5.5, 60°C | Rhizomucor miehei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Rhizomucor miehei | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31564 | - |
1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation | Rhizomucor miehei |
33700 | - |
gel filtration | Rhizomucor miehei |
35400 | - |
1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation | Rhizomucor miehei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizomucor miehei | H9BZ70 | gene RmLic16A | - |
Rhizomucor miehei CAU432 | H9BZ70 | gene RmLic16A | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains two possible N-glycosylation sites at 72 (NKT) and 275 (NPT), respectively | Rhizomucor miehei |
Purification (Comment) | Organism |
---|---|
native enzyme 6.8fold by ammonium sulfate fractionation and two different steps of anion exchange chromatography, to homogeneity | Rhizomucor miehei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | high-level extracellular enzyme production of 6230 U/mL on oat flour at 3% w/v as a carbon source at 50 °C | Rhizomucor miehei | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28820 | 33480 | purified native enzyme, substrate barley beta-glucan, pH 5.5, 60°C | Rhizomucor miehei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | best substrate | Rhizomucor miehei | cellotriose + cellotetraose + cellobiose + D-glucose | oligosaccharides, mainly trisaccharide and tetrasaccharide | ? | |
barley beta-glucan + H2O | best substrate | Rhizomucor miehei CAU432 | cellotriose + cellotetraose + cellobiose + D-glucose | oligosaccharides, mainly trisaccharide and tetrasaccharide | ? | |
lichenan + H2O | 95.2% activity compared to barley beta-glucan | Rhizomucor miehei | cellotriose + ? | oligosaccharides, mainly trisaccharide | ? | |
lichenan + H2O | 95.2% activity compared to barley beta-glucan | Rhizomucor miehei CAU432 | cellotriose + ? | oligosaccharides, mainly trisaccharide | ? | |
additional information | thin layer chromatography product analysis, overview. Poor activity with laminarin, no activity with birchwood xylan, carboxymethyl cellulose, pullulan, soluble starch, locust bean gum, cellulose, Avicel, beta-1,3-glucan, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-galactopyranoside, substrate specificity, overview | Rhizomucor miehei | ? | - |
? | |
additional information | thin layer chromatography product analysis, overview. Poor activity with laminarin, no activity with birchwood xylan, carboxymethyl cellulose, pullulan, soluble starch, locust bean gum, cellulose, Avicel, beta-1,3-glucan, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-galactopyranoside, substrate specificity, overview | Rhizomucor miehei CAU432 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation | Rhizomucor miehei |
More | peptide mapping of native enzyme | Rhizomucor miehei |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Rhizomucor miehei |
endo-beta-1,3-1,4-glucanase | - |
Rhizomucor miehei |
Lichenase | - |
Rhizomucor miehei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Rhizomucor miehei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | activity range, profile overview | Rhizomucor miehei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified native enzyme, no loss in activity at pH 5.5 for 30 min, half-life is 225 min | Rhizomucor miehei |
70 | - |
purified native enzyme, pH 5.5, half-life is 101 min | Rhizomucor miehei |
75 | - |
purified native enzyme, pH 5.5, half-life is 64 min | Rhizomucor miehei |
80 | - |
purified native enzyme, pH 5.5, half-life is 53 min, 80% activity remaining after 30 min | Rhizomucor miehei |
85 | - |
purified native enzyme, pH 5.5, half-life is 27 min, inactivation after 120 min | Rhizomucor miehei |
90 | - |
purified native enzyme, pH 5.5, 60% activity remaining after 30 min | Rhizomucor miehei |
100 | - |
purified native enzyme, pH 5.5, 40% activity remaining after 30 min | Rhizomucor miehei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Rhizomucor miehei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7.5 | activity range, profile overview | Rhizomucor miehei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | purified native enzyme, retains 90% of its activity at 60°C for 30 min | Rhizomucor miehei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhizomucor miehei | sequence calculation | - |
3.9 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the glycosylhydrolase faamily GH16 | Rhizomucor miehei |