Cloned (Comment) | Organism |
---|---|
plasmid carrying the truncated 1,3-1,4-beta-D-glucanase gene in the pET26b(+) vector, transformed into Escherichia coli XL1-Blue. Overexpression of the truncated form and mutants in Escherichia coli BL21 (DE3) cells | Fibrobacter succinogenes |
Crystallization (Comment) | Organism |
---|---|
mutant E85I, at 2.2 A resolution, by hanging-drop vapour-diffusion method at room temperature. The mutant crystallizes in space group P3121 with one molecule in the asymmetric unit, Ca2+ ion-binding site is maintained | Fibrobacter succinogenes |
Protein Variants | Comment | Organism |
---|---|---|
E85D | has 5fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
E85I | has 5fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
N72A | has 11fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
N72Q | has 17fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
Q70A | has 299fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
Q70D | has 62fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
Q70E | has 106fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
Q70I | has 499fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
Q70N | has 63fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
Q70R | has 35fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
Q81I | has 2fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
Q81N | has 2.5fold lower kcat/Km ratios than the wild-type | Fibrobacter succinogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Fibrobacter succinogenes | |
Cs2+ | - |
Fibrobacter succinogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fibrobacter succinogenes | P17989 | - |
- |
Purification (Comment) | Organism |
---|---|
mutants purified on Ni-NTA affinity column, more than 95% purity | Fibrobacter succinogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
236 | - |
mutant Q70I | Fibrobacter succinogenes |
527 | - |
mutant Q70A | Fibrobacter succinogenes |
802 | - |
mutant Q70N | Fibrobacter succinogenes |
1089 | - |
mutant N72Q | Fibrobacter succinogenes |
1381 | - |
mutant Q70D | Fibrobacter succinogenes |
1385 | - |
mutant Q70E | Fibrobacter succinogenes |
1699 | - |
mutant E85I | Fibrobacter succinogenes |
1774 | - |
mutant N72A | Fibrobacter succinogenes |
3076 | - |
mutant E85D | Fibrobacter succinogenes |
3104 | - |
mutant Q70R | Fibrobacter succinogenes |
5758 | - |
mutant Q81N | Fibrobacter succinogenes |
7873 | - |
mutant Q81I | Fibrobacter succinogenes |
8478 | - |
truncated enzyme | Fibrobacter succinogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lichenan + H2O | - |
Fibrobacter succinogenes | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,3-1,4-beta-D-glucanase | - |
Fibrobacter succinogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
109 | - |
lichenan | mutant Q70I, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
244 | - |
lichenan | mutant Q70A, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
371 | - |
lichenan | mutant Q70N, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
504 | - |
lichenan | mutant N72Q, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
639 | - |
lichenan | mutant Q70D, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
641 | - |
lichenan | mutant Q70E, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
785 | - |
lichenan | mutant E85I, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
820 | - |
lichenan | mutant N72A, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
1422 | - |
lichenan | mutant E85D, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
1435 | - |
lichenan | mutant Q70R, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
2663 | - |
lichenan | mutant Q81N, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
3641 | - |
lichenan | mutant Q81I, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
3920 | - |
lichenan | truncated enzyme, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes |