Literature summary for 3.2.1.7 extracted from

Guo, N.; Gong, F.; Chi, Z.; Sheng, J.; Li, J.
Enhanced inulinase production in solid state fermentation by a mutant of the marine yeast Pichia guilliermondii using surface response methodology and inulin hydrolysis (2009), J. Ind. Microbiol. Biotechnol., 36, 499-507.

Search for more literature for 3.2.1.7

Application

Application	Comment	Organism
industry	inulinase activity (115.0 U/ml) in liquid production medium by the mutant M-30 is the highest reported so far. Inulinase produced by the mutant M-30 of the marine yeast strain has potentially for use in biotechnological fields	Meyerozyma guilliermondii

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and mutant M-30 PCR products ligated into pGEM-T easy vector and transformed into the competent cells of Escherichia coli JM109	Meyerozyma guilliermondii

Protein Variants

Protein Variants	Comment	Organism
additional information	mutants M-30 (115.0 U/ml) and M-31 (52.6 U/ml) show improved inulinase production compared to the wild-type. Initial moisture, inoculum, amount ratio of wheat bran to rice bran, temperature, pH for the maximum inulinase production by the mutant M-30 is 60.5%, 2.5%, 0.42, 30°C and 6.50, respectively. Under the optimized conditions, 455.9 U/grams of dry substrate (gds) of inulinase activity is reached in the solid state fermentation culture of the mutant. Glucose repression on the inulinase production by the mutant M-30 is relieved in some degree compared to that in its parent strain when the added glucose concentrations in the media are higher than 2.0%	Meyerozyma guilliermondii

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	glucose represses inulinase production	Meyerozyma guilliermondii

Organism

Organism	UniProt	Comment	Textmining
Meyerozyma guilliermondii	A5DHM6	-	-
Meyerozyma guilliermondii 1	A5DHM6	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and mutant M-30	Meyerozyma guilliermondii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
inulin + H2O	-	Meyerozyma guilliermondii	?	-	?
inulin + H2O	-	Meyerozyma guilliermondii 1	?	-	?

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Release 2023.1 (January 2023)