Cloned (Comment) | Organism |
---|---|
gene ACM61449, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain origami(DE3) | Caldicellulosiruptor bescii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 68% inhibition at 1 mM | Caldicellulosiruptor bescii | |
Ca2+ | 48% inhibition at 1 mM | Caldicellulosiruptor bescii | |
Cd2+ | complete inhibition at 1 mM | Caldicellulosiruptor bescii | |
Cu2+ | complete inhibition at 1 mM | Caldicellulosiruptor bescii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, recombinant His-tagged enzyme, SDS-PAGE, x * 51000, about, sequence calculation of mature enzyme | Caldicellulosiruptor bescii |
51000 | - |
x * 50000, recombinant His-tagged enzyme, SDS-PAGE, x * 51000, about, sequence calculation of mature enzyme | Caldicellulosiruptor bescii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-alpha-D-galacturonide]n + H2O | Caldicellulosiruptor bescii | the enzyme releases monogalacturonic acid from its nonreducing end | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | Caldicellulosiruptor bescii DSM 6725 | the enzyme releases monogalacturonic acid from its nonreducing end | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldicellulosiruptor bescii | - |
gene ACM61449 | - |
Caldicellulosiruptor bescii DSM 6725 | - |
gene ACM61449 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain origami(DE3) by heat treatment for 30 min at 70°C, and nickel affinity chromatography | Caldicellulosiruptor bescii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is specifically active towards the alpha-1,4-galacturonic acid linkages of galactopolysaccharides, no activity on Avicel, carboxymethyl cellulose, xylan, glucan, and soluble starch. A higher degree of pectin methylation corresponds to a lower activity of the enzyme | Caldicellulosiruptor bescii | ? | - |
? | |
additional information | the enzyme is specifically active towards the alpha-1,4-galacturonic acid linkages of galactopolysaccharides, no activity on Avicel, carboxymethyl cellulose, xylan, glucan, and soluble starch. A higher degree of pectin methylation corresponds to a lower activity of the enzyme | Caldicellulosiruptor bescii DSM 6725 | ? | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | the enzyme releases monogalacturonic acid from its nonreducing end | Caldicellulosiruptor bescii | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | the enzyme releases monogalacturonic acid from its nonreducing end, galacturonic acid is the only product of degradation | Caldicellulosiruptor bescii | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | the enzyme releases monogalacturonic acid from its nonreducing end | Caldicellulosiruptor bescii DSM 6725 | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | the enzyme releases monogalacturonic acid from its nonreducing end, galacturonic acid is the only product of degradation | Caldicellulosiruptor bescii DSM 6725 | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, recombinant His-tagged enzyme, SDS-PAGE, x * 51000, about, sequence calculation of mature enzyme | Caldicellulosiruptor bescii |
Synonyms | Comment | Organism |
---|---|---|
CbPelA | - |
Caldicellulosiruptor bescii |
EPG | - |
Caldicellulosiruptor bescii |
exo-polygalacturonase | - |
Caldicellulosiruptor bescii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
72 | - |
- |
Caldicellulosiruptor bescii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 75 | over 90% of maximal activity within this range | Caldicellulosiruptor bescii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified recombinant His-tagged enzyme, half-life is 14 h | Caldicellulosiruptor bescii |
80 | - |
purified recombinant His-tagged enzyme, half-life is 90 min | Caldicellulosiruptor bescii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
- |
Caldicellulosiruptor bescii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | purified recombinant His-tagged enzyme, 80% activity remaining | Caldicellulosiruptor bescii |
General Information | Comment | Organism |
---|---|---|
physiological function | the polygalacturonase catalyzes the hydrolysis of the homo-galacturonan skeleton | Caldicellulosiruptor bescii |