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Literature summary for 3.2.1.64 extracted from

  • Song, E.K.; Kim, H.; Sung, H.K.; Cha, J.
    Cloning and characterization of a levanbiohydrolase from Microbacterium laevaniformans ATCC 15953 (2002), Gene, 291, 45-55.
    View publication on PubMed
Search for more literature for 3.2.1.64

Cloned(Commentary)

Cloned (Comment) Organism
-
 Microbacterium laevaniformans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Microbacterium laevaniformans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68800
-
 x * 68800, SDS-PAGE Microbacterium laevaniformans

Organism

Organism UniProt Comment Textmining
Microbacterium laevaniformans
-
 strain ATCC 15953
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, expression n Escherichia coli Microbacterium laevaniformans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
997.3
-
 pH 6.0, 30°C Microbacterium laevaniformans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O 0.2% of the activity with levan from Serratia sp. Microbacterium laevaniformans ?
-
 ?
inulin + H2O 5% of the activity with levan from Serratia sp. Microbacterium laevaniformans ?
-
 ?
levan + H2O enzyme activity depends on source of levan, sources in decreasing order of activity: Serratia sp., Rhanella aquatilis, Zymomonas mobilis, M. laevaniformans Microbacterium laevaniformans levanbiose + ?
-
 ?
additional information enzyme cleaves beta-2,6-linkage of levan and beta-2,1-linkage of fructooligosaccharides, no substrate are: soluble starch, cellobiose, sucrose, maltose, melibiose, raffinose, lactose Microbacterium laevaniformans ?
-
 ?
nystose + H2O 0.5% of the activity with levan from Serratia sp. Microbacterium laevaniformans ?
-
 ?

Subunits

Subunits Comment Organism
? x * 68800, SDS-PAGE Microbacterium laevaniformans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Microbacterium laevaniformans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, 10% loss of activity Microbacterium laevaniformans
50
-
 30 min, 65% loss of activiy Microbacterium laevaniformans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Microbacterium laevaniformans

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 more than 80% of maximum activity in this range Microbacterium laevaniformans