Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Origami B(DE3) cells | Corallococcus sp. EGB |
Protein Variants | Comment | Organism |
---|---|---|
E304A | inactive | Corallococcus sp. EGB |
E309A | inactive | Corallococcus sp. EGB |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | Corallococcus sp. EGB | most favorable substrate | D-glucose + ? | - |
? | |
pachyman + H2O | Corallococcus sp. EGB | - |
D-glucose + ? | - |
? | |
pustulan + H2O | Corallococcus sp. EGB | - |
D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corallococcus sp. EGB | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Corallococcus sp. EGB |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | most favorable substrate | Corallococcus sp. EGB | D-glucose + ? | - |
? | |
pachyman + H2O | - |
Corallococcus sp. EGB | D-glucose + ? | - |
? | |
pustulan + H2O | - |
Corallococcus sp. EGB | D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47040, calculated from amino acid sequence | Corallococcus sp. EGB |
Synonyms | Comment | Organism |
---|---|---|
beta-(1,3)(1,3)-glucanase | - |
Corallococcus sp. EGB |
beta-(1,3)-glucanase | - |
Corallococcus sp. EGB |
lamC | - |
Corallococcus sp. EGB |
lamC | the enzyme shows exo-beta-(1,3)/(1,6)-glucanase and displays and endo-beta-(1,4)-glucanase/xylanase activities | Corallococcus sp. EGB |
laminarinase | - |
Corallococcus sp. EGB |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Corallococcus sp. EGB |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme shows about 70% activity at 30°C, about 75% activity at 35°C, about 85% activity at 40°C, about 90% activity at 45°C, 100% activity at 50°C, about 88% activity at 55°C, about 65% activity at 60°C, about 40% activity at 65°C, and about 25% activity at 70°C | Corallococcus sp. EGB |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme retains more than 80% of its original activity after 30 min of incubation at 50°C | Corallococcus sp. EGB |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Corallococcus sp. EGB |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme shows more than 75% of activity between pH 6.0 and 8.0 | Corallococcus sp. EGB |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme retains more than 60% of its original activity after 30 min of incubation at pH 6.0-8.0 | Corallococcus sp. EGB |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Corallococcus sp. EGB | isoelectric focusing | - |
5.3 |