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Literature summary for 3.2.1.58 extracted from
- Major change in regiospecificity for the exo-1,3-beta-glucanase from Candida albicans following its conversion to a glycosynthase (2014), Biochemistry, 53, 3318-3326.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant mutant E282S alone or in complex with alpha-D-glucopyranosyl fluoride and 4-nitrophenyl-beta-D-glucopyranoside, or with 4-nitrophenyl-gentiobiose, X-ray diffraction structure determination and analysis at 1.59-1.96 A resolution | Candida albicans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E292G | site-directed mutagenesis | Candida albicans |
| E292S | site-directed mutagenesis, the mutant shows altered transglycosidase activity as the wild-type enzyme and displays modest glycosynthase activity with alpha-D-glucopyranosyl fluoride as the donor and 4-nitrophenyl-beta-D-glucopyranoside as the acceptor, but surprisingly shows a marked preference for synthesizing beta-1,6-linked over beta-1,3- and beta-1,4-linked disaccharide products. Mechanism and expected regiospecificity for the glycosynthase reaction, overview | Candida albicans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | P29717 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme shows glycohydrolase and transglycosidase activities, substrate binding structures determined from mutant E292S complex crystal structures, detailed overview | Candida albicans | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Exg | - |
Candida albicans |
| exo-1,3-beta-glucanase | - |
Candida albicans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Candida albicans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in cell wall beta-D-glucan metabolism and morphogenesis through its hydrolase and transglycosidase activities | Candida albicans |
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Release 2023.1 (January 2023)
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