Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Geobacillus stearothermophilus | |
Fe3+ | slight inhibition | Geobacillus stearothermophilus | |
Hg2+ | - |
Geobacillus stearothermophilus | |
Pb2+ | - |
Geobacillus stearothermophilus | |
Zn2+ | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.67 | - |
beta-cyclodextrin | - |
Geobacillus stearothermophilus | |
1.79 | - |
alpha-cyclodextrin | - |
Geobacillus stearothermophilus | |
2.5 | - |
gamma-cyclodextrin | - |
Geobacillus stearothermophilus | |
8.3 | - |
pullulan | - |
Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | increase activity to 112%/102% | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
- |
Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
HY-1 | - |
Geobacillus stearothermophilus HY-1 | - |
HY-1 | - |
Purification (Comment) | Organism |
---|---|
purified 230fold | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.4 | - |
affinity chromatography | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin | - |
Geobacillus stearothermophilus | maltotriose + maltose + glucose | - |
? | |
alpha-cyclodextrin | - |
Geobacillus stearothermophilus HY-1 | maltotriose + maltose + glucose | - |
? | |
amylopectin + H2O | - |
Geobacillus stearothermophilus | maltose + ? | - |
? | |
amylose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
amylose + H2O | - |
Geobacillus stearothermophilus HY-1 | ? | - |
? | |
beta-cyclodextrin + H2O | - |
Geobacillus stearothermophilus | maltose + D-glucose | - |
? | |
cyclomaltodextrin + H2O | - |
Geobacillus stearothermophilus | maltodextrin | - |
? | |
cyclomaltodextrin + H2O | - |
Geobacillus stearothermophilus HY-1 | maltodextrin | - |
? | |
gamma-cyclodextrin + H2O | - |
Geobacillus stearothermophilus | maltose + D-glucose | - |
? | |
maltodextrins + H2O | linear, enzyme degrades the linear molecules by splitting off maltose units from the nonreducing end of the chain | Geobacillus stearothermophilus | maltose + glucose | - |
? | |
maltodextrins + H2O | linear, enzyme degrades the linear molecules by splitting off maltose units from the nonreducing end of the chain | Geobacillus stearothermophilus HY-1 | maltose + glucose | - |
? | |
pullulan + H2O | - |
Geobacillus stearothermophilus | panose + maltose + glucose | - |
? | |
starch + H2O | - |
Geobacillus stearothermophilus | glucose + maltose | - |
? | |
starch + H2O | - |
Geobacillus stearothermophilus HY-1 | glucose + maltose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | - |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable at temperatures below | Geobacillus stearothermophilus |
60 | - |
- |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | - |
Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7.6 | - |
Geobacillus stearothermophilus |