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Literature summary for 3.2.1.52 extracted from

  • Lv, C.; Gu, T.; Xu, K.; Gu, J.; Li, L.; Liu, X.; Zhang, A.; Gao, S.; Li, W.; Zhao, G.
    Biochemical characterization of a beta-N-acetylhexosaminidase from Streptomyces alfalfae and its application in the production of N-acetyl-D-glucosamine (2019), J. Biosci. Bioeng., 128, 135-141 .
    View publication on PubMed
Search for more literature for 3.2.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces alfalfae

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 25% residual activity at 1 mM Streptomyces alfalfae
Ca2+ 92% residual activity at 1 mM Streptomyces alfalfae
Cu2+ 94% residual activity at 1 mM Streptomyces alfalfae
EDTA 91% residual activity at 1 mM Streptomyces alfalfae
Fe2+ 72% residual activity at 1 mM Streptomyces alfalfae
Hg2+ 5% residual activity at 1 mM Streptomyces alfalfae
Zn2+ 56% residual activity at 1 mM Streptomyces alfalfae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 104% activity at 1 mM Streptomyces alfalfae
Fe3+ 129% activity at 1 mM Streptomyces alfalfae
Mg2+ 121% activity at 1 mM Streptomyces alfalfae
Mn2+ 111% activity at 1 mM Streptomyces alfalfae
additional information not influenced by Ni2+, K+, and Na+ Streptomyces alfalfae

Organism

Organism UniProt Comment Textmining
Streptomyces alfalfae A0A3G3NI51
-
-
Streptomyces alfalfae ACCC40021 A0A3G3NI51
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose column chromatography Streptomyces alfalfae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
215
-
 with N-acetylchitobiose as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
234.9
-
 with N-acetylchitotriose as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
242.6
-
 with N-acetylchitohexaose as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
253.9
-
 with N-acetylchitopentaose as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
395.2
-
 with N-acetylchitotetraose as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
491
-
 with 4-nitrophenyl N-acetyl-beta-D-galactosaminide as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
1149.7
-
 with 4-nitrophenyl N-acetyl-beta-D-glucosaminide as substrate, at pH 5.5 and 60°C Streptomyces alfalfae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O 42.7% activity compared to 4-nitrophenyl N-acetyl-beta-D-glucosaminide Streptomyces alfalfae 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O 42.7% activity compared to 4-nitrophenyl N-acetyl-beta-D-glucosaminide Streptomyces alfalfae ACCC40021 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O 100% activity Streptomyces alfalfae 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O 100% activity Streptomyces alfalfae ACCC40021 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
crab shell a-chitin + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine
-
 ?
crab shell a-chitin + H2O
-
 Streptomyces alfalfae ACCC40021 N-acetyl-beta-D-glucosamine
-
 ?
N-acetylchitobiose + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine
-
 ?
N-acetylchitobiose + H2O
-
 Streptomyces alfalfae ACCC40021 N-acetyl-beta-D-glucosamine
-
 ?
N-acetylchitohexaose + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine + N-acetylchitobiose + N-acetylchitotriose + N-acetylchitotetraose + N-acetylchitopentaose
-
 ?
N-acetylchitohexaose + H2O
-
 Streptomyces alfalfae ACCC40021 N-acetyl-beta-D-glucosamine + N-acetylchitobiose + N-acetylchitotriose + N-acetylchitotetraose + N-acetylchitopentaose
-
 ?
N-acetylchitopentaose + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine + N-acetylchitobiose + N-acetylchitotriose + N-acetylchitotetraose
-
 ?
N-acetylchitotetraose + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine + N-acetylchitobiose + N-acetylchitotriose
-
 ?
N-acetylchitotriose + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine + N-acetylchitobiose
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Streptomyces alfalfae
? x * 55664, calculated from amino acid sequence Streptomyces alfalfae

Synonyms

Synonyms Comment Organism
exo-type beta-N-acetylhexosaminidase
-
 Streptomyces alfalfae
Hex
-
 Streptomyces alfalfae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces alfalfae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 65 more than 80% of the maximum activity at 60-65°C Streptomyces alfalfae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 50 the enzyme is thermo-stable up to 45°C, retaining 85.3% activity even at 50°C Streptomyces alfalfae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Streptomyces alfalfae

pH Range

pH Minimum pH Maximum Comment Organism
5.9 6 more than 80% of the maximum activity at pH 5.0-6.0 Streptomyces alfalfae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 8.5 the enzyme retains stable at pH 4.5-8.5, with more than 80 % of activity after 1 h-incubation Streptomyces alfalfae

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces alfalfae calculated from amino acid sequence
-
 7.9