Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Glycopeptide | dimeric | Escherichia coli |
General Stability | Organism |
---|---|
enzyme unstable, extensive purification only in presence of 20% glycerol possible | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Escherichia coli | |
2.58 | - |
glycopeptide monomer | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33500 | - |
gel filtration | Escherichia coli |
36000 | - |
1 * 36000, SDS-PAGE | Escherichia coli |
38000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Escherichia coli | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1094fold to near homogeneity | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
162.3 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, in presence of 20% glycerol stable for at least 2 months, even more stable in 50% glycerol | Escherichia coli |
-70°C, in presence of 20% glycerol stable for at least 3 months, even more stable in 50% glycerol | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycopeptide dimer + H2O | - |
Escherichia coli | N-acetylglucosamine + ? | - |
? | |
glycopeptide monomer + H2O | - |
Escherichia coli | N-acetylglucosamine + ? | - |
? | |
murein-lipoprotein complex + H2O | - |
Escherichia coli | N-acetylglucosamine + ? | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Escherichia coli | N-acetyl-D-hexosamines | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Escherichia coli | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
crude enzyme preparation | Escherichia coli |
7.7 | - |
purified enzyme | Escherichia coli |