Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(lambdaDE3) cells | Bifidobacterium bifidum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM Cu2+ reduces the activity to 10% | Bifidobacterium bifidum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bifidobacterium bifidum | - |
- |
membrane | a membrane-anchored extracellular enzyme | Bifidobacterium bifidum | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+, Mg2+, Zn2+, Mn2+, Ni2+, and Co2+, do not affect enzyme activity at 5 mM | Bifidobacterium bifidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium bifidum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, and Superdex 200 gel filtration | Bifidobacterium bifidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl 4-O-N-acetyl-alpha-D-glucosaminyl-beta-D-galactoside + H2O | - |
Bifidobacterium bifidum | N-acetyl-alpha-D-glucosamine + 4-methylumbelliferyl beta-D-galactosaminide | - |
? | |
4-methylumbelliferyl 6-O-N-acetyl-alpha-D-glucosaminyl-beta-D-galactoside + H2O | slow reaction | Bifidobacterium bifidum | N-acetyl-alpha-D-glucosamine + 4-methylumbelliferyl beta-D-galactosaminide | - |
? | |
4-methylumbelliferyl N-acetyl-alpha-D-glucosaminide + H2O | efficient substrate | Bifidobacterium bifidum | 4-methylumbelliferone + N-acetyl-alpha-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-alpha-D-glucosaminide + H2O | slow reaction | Bifidobacterium bifidum | 4-nitrophenol + N-acetyl-alpha-D-glucosamine | - |
? | |
additional information | no activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide, 4-nitrophenyl N-acetyl-alpha-D-galactosaminide, 4-nitrophenyl N-acetyl-beta-D-galactosaminide, 4-nitrophenyl alpha-D-glucosaminide, 4-nitrophenyl beta-D-glucosaminide, 4-nitrophenyl alpha-D-galactosaminide, 4-nitrophenyl beta-D-galactosaminide, 4-nitrophenyl alpha-D-fucosaminide, 4-nitrophenyl beta-D-fucosaminide, 4-nitrophenyl alpha-D-mannosaminide, 4-nitrophenyl beta-D-mannosaminide, 4-nitrophenyl alpha-D-xylosaminide, and 4-nitrophenyl beta-D-xylosaminide. Alpha1,2- and alpha1,3-linked N-acetyl-alpha-D-glucosaminides are resistant to this enzyme | Bifidobacterium bifidum | ? | - |
? | |
porcine gastric mucin + H2O | the enzyme specifically hydrolyzes alpha-linked N-acetylglucosamine at nonreducing terminus of O-glycan on gastric mucin | Bifidobacterium bifidum | N-acetyl-alpha-D-glucosamine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 200000, SDS-PAGE | Bifidobacterium bifidum |
? | x * 212000, calculated from amino acid sequence | Bifidobacterium bifidum |
Synonyms | Comment | Organism |
---|---|---|
AgnB | - |
Bifidobacterium bifidum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bifidobacterium bifidum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the enzyme is stable up to 40°C | Bifidobacterium bifidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Bifidobacterium bifidum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | more than 80 % activity is detected between pH 4.0 and 6.5 | Bifidobacterium bifidum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | the enzyme is stable between pH 5.0 and 9.5 | Bifidobacterium bifidum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is important for assimilation of gastroduodenal mucin by Bacillus bifidum and also applicable to production of prebiotic oligosaccharides from porcine gastric mucin | Bifidobacterium bifidum |