Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-acetylglucosamine | competitive inhibition | Sus scrofa | |
N-acetylglucosaminolactone | competitive inhibition | Sus scrofa | |
N-acetylmannosamine | competitive inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
o-nitrophenyl-alpha-N-acetylglucosaminide | 0.08 partially purified enzyme | Sus scrofa | |
0.3 | - |
p-nitrophenyl-alpha-N-acetylglucosaminide | partially purified enzyme | Sus scrofa | |
1.4 | - |
phenyl-alpha-N-acetylglucosaminide | partially purified enzyme | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Sus scrofa | 5764 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 82% inhibition by 1 mM CuSO4 | Sus scrofa | |
Fe3+ | 76% inhibition by 1 mM ferric chloride | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(GlcNAcalpha(1-4))n + H2O | Sus scrofa | - |
(GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Rattus norvegicus | - |
brain | - |
Rattus norvegicus | - |
duodenum | - |
Rattus norvegicus | - |
epididymis | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
jejunum | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
lung | - |
Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
skeletal muscle | - |
Rattus norvegicus | - |
skin | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
testis | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.027 | - |
partially purified enzyme | Sus scrofa |
Storage Stability | Organism |
---|---|
4°C, pH 6, crude enzyme, several months, no loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(GlcNAcalpha(1-4))n + H2O | - |
Sus scrofa | (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | - |
? | |
o-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O | - |
Sus scrofa | o-nitrophenol + N-acetyl-alpha-D-glucosamine | - |
? | |
p-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O | - |
Sus scrofa | p-nitrophenol + N-acetyl-alpha-D-glucosamine | - |
? | |
phenyl-N-acetyl-alpha-D-glucosaminide + H2O | - |
Sus scrofa | phenol + N-acetyl-alpha-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-acetamido-2-deoxy-alpha-D-glucoside acetamidodeoxyglucohydrolase | - |
Sus scrofa |
alpha-acetylglucosaminidase | - |
Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
phenyl-alpha-N-acetylglucosaminide | Sus scrofa |
4.7 | - |
p-nitrophenyl-alpha-N-acetylglucosaminide | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
not stable below 4°C | Sus scrofa |