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Literature summary for 3.2.1.26 extracted from

  • Sun, Z.; Du, Y.; Yang, F.; Wang, X.; Wang, Y.; Li, H.; Li, R.; Tang, W.; Li, X.
    Identification of an active-site residue in invertase SUC2 by mass spectrometry-based proteomics and site-directed mutagenesis (2016), Int. J. Mass Spectrom., 409, 9-15 .
No PubMed abstract available
Search for more literature for 3.2.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris X-33 and Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
K185R mutant enzyme K185R shows higher stability than wild-type enzyme at 40°C-50°C. These results suggest that the deubiquitination of K185 slightly affects the thermal stability of SUC2 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 enzyme expressed in Escherichia coli, SDS-PAGE Saccharomyces cerevisiae
85000
-
 enzyme expressed in Pichia pastoris, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P00724
-
-
Saccharomyces cerevisiae ATCC 204508 P00724
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ubiquitination invertase SUC2 is ubiquitinated at three lysine residue sites (K185, K312 and K430), ubiquitination of SUC2 in the site of 185 contributes to the high enzyme activity. Deubiquitination of K185 slightly affects the thermal stability of SUC2 Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
 Saccharomyces cerevisiae beta-D-fructose + ?
-
 ?
inulin + H2O
-
 Saccharomyces cerevisiae ATCC 204508 beta-D-fructose + ?
-
 ?
sucrose + H2O
-
 Saccharomyces cerevisiae beta-D-fructose + alpha-D-glucose
-
 ?
sucrose + H2O
-
 Saccharomyces cerevisiae ATCC 204508 beta-D-fructose + alpha-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
invertase SUC2
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 mutant enzyme K185R shows higher stability than wild-type enzyme at 40°C-50°C Saccharomyces cerevisiae