Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bifidobacterium longum |
Crystallization (Comment) | Organism |
---|---|
apo form of beta-fructofuranosidase and its complex with fructose, both to 1.8 A resolution. Enzyme consists of the N-terminal five-blade beta-propeller domain that includes the catalytic site, as well as the beta-sandwich C-terminal domain. The active site is located at the bottom of the axial funnel created by the interblade loops and the loops between beta-strand 2-3 of each blade. The protein has a 35-residue elongation of the N-terminus containing a fiveturn alpha-helix, which distinguishes it from the other known members of the GH32 family | Bifidobacterium longum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59156 | - |
x * 59156, calculated for recombinant protein. x * 60000, SDS-PAGE | Bifidobacterium longum |
60000 | - |
x * 59156, calculated for recombinant protein. x * 60000, SDS-PAGE | Bifidobacterium longum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium longum | A2TLS9 | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 59156, calculated for recombinant protein. x * 60000, SDS-PAGE | Bifidobacterium longum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bifidobacterium longum | calculated | - |
4.9 |