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Literature summary for 3.2.1.26 extracted from
- Molecular cloning and functional identification of invertase isozymes from green bamboo Bambusa oldhamii (2006), J. Agric. Food Chem., 54, 3101-3107.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme betafruct1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, very poor expression of His-tagged isozyme betafruct1 in Pichia pastoris strain YIT1 | Bambusa oldhamii |
| isozyme betafruct2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged isozyme betafruct2 in Pichia pastoris strain YIT2 | Bambusa oldhamii |
| isozyme betafruct3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged isozyme betafruct3 in Pichia pastoris strain YIT3 | Bambusa oldhamii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | cell wall specific isozyme betafruct1 | Bambusa oldhamii | 5618 | - |
| cell wall | cell wall specific isozyme betafruct2 | Bambusa oldhamii | 5618 | - |
| cell wall | cell wall specific isozyme betafruct3 | Bambusa oldhamii | 5618 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68200 | - |
1 * 77500, recombinant His-tagged isozyme betafruct2, SDS-PAGE, 1 * 68200, deglycosylated recombinant His-tagged isozyme betafruct2, SDS-PAGE | Bambusa oldhamii |
| 68200 | - |
1 * 77500, recombinant His-tagged isozyme betafruct3, SDS-PAGE, 1 * 68200, deglycosylated recombinant His-tagged isozyme betafruct3, SDS-PAGE | Bambusa oldhamii |
| 70100 | - |
recombinant His-tagged isozyme betafruct2, gel filtration | Bambusa oldhamii |
| 72600 | - |
recombinant His-tagged isozyme betafruct3, gel filtration | Bambusa oldhamii |
| 77500 | - |
1 * 77500, recombinant His-tagged isozyme betafruct2, SDS-PAGE, 1 * 68200, deglycosylated recombinant His-tagged isozyme betafruct2, SDS-PAGE | Bambusa oldhamii |
| 77500 | - |
1 * 77500, recombinant His-tagged isozyme betafruct3, SDS-PAGE, 1 * 68200, deglycosylated recombinant His-tagged isozyme betafruct3, SDS-PAGE | Bambusa oldhamii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bambusa oldhamii | Q2XQ19 | isozyme betafruct3; three isozymes betafruct1-3 | - |
| Bambusa oldhamii | Q2XQ20 | isozyme betafruct2; three isozymes betafruct1-3 | - |
| Bambusa oldhamii | Q2XQ21 | isozyme betafruct1; three isozymes betafruct1-3 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | isozyme betafruct1 contains 3 potential glycosylation sites | Bambusa oldhamii |
| glycoprotein | isozyme betafruct2 contains 3 potential glycosylation sites | Bambusa oldhamii |
| glycoprotein | isozyme betafruct3 contains 4 potential glycosylation sites | Bambusa oldhamii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged isozyme betafruct2 15.1fold from Pichia pastoris strain YIT2 by ammonium sulfate fractionation and cobalt affinity chromatography | Bambusa oldhamii |
| recombinant His-tagged isozyme betafruct3 24.3fold from Pichia pastoris strain YIT3 by ammonium sulfate fractionation and cobalt affinity chromatography | Bambusa oldhamii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 884.3 | - |
purified recombinant His-tagged isozyme betafruct2 | Bambusa oldhamii |
| 1695 | - |
purified recombinant His-tagged isozyme betafruct3 | Bambusa oldhamii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + H2O | - |
Bambusa oldhamii | D-glucose + D-fructose | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 77500, recombinant His-tagged isozyme betafruct2, SDS-PAGE, 1 * 68200, deglycosylated recombinant His-tagged isozyme betafruct2, SDS-PAGE | Bambusa oldhamii |
| monomer | 1 * 77500, recombinant His-tagged isozyme betafruct3, SDS-PAGE, 1 * 68200, deglycosylated recombinant His-tagged isozyme betafruct3, SDS-PAGE | Bambusa oldhamii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| invertase | - |
Bambusa oldhamii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant His-tagged isozyme betafruct3 | Bambusa oldhamii |
| 60 | - |
recombinant His-tagged isozyme betafruct2 | Bambusa oldhamii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant His-tagged isozyme betafruct2, complete loss of activity | Bambusa oldhamii |
| 50 | - |
recombinant His-tagged isozyme betafruct3, complete loss of activity | Bambusa oldhamii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
recombinant His-tagged isozyme betafruct2 | Bambusa oldhamii |
| 4 | 5 | recombinant His-tagged isozyme betafruct3 | Bambusa oldhamii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 4.5 | recombinant His-tagged isozyme betafruct2, about 90% of maximal activity at pH 2.0 and 20% at pH 4.5 | Bambusa oldhamii |
| 3.5 | 6 | recombinant His-tagged isozyme betafruct3, about 50% of maximal activity at pH 3.5 and pH 6.0 | Bambusa oldhamii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH stability profile | Bambusa oldhamii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bambusa oldhamii | isozyme betafruct3, amino acid sequence calculation | - |
5.7 |
| Bambusa oldhamii | recombinant His-tagged isozyme betafruct3, isoelectric focusing | - |
6.5 |
| Bambusa oldhamii | isozyme betafruct2, amino acid sequence calculation | - |
7.3 |
| Bambusa oldhamii | isozyme betafruct1, amino acid sequence calculation | - |
8.9 |
| Bambusa oldhamii | recombinant His-tagged isozyme betafruct2, isoelectric focusing | - |
9.2 |
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