Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
D206N | in contrast to the wild-type enzyme which shows a catalytic efficiency for 4-nitrophenyl beta-D-mannopyranoside that is 5fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 4fold higher than that for 4-nitrophenyl beta-D-glucopyranoside, the mutant enzyme shows highest catalytic efficiency towards 4-nitrophenyl beta-D-glucopyranoside, 11fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 60fold higher than that for 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus horikoshii |
Q77R | in contrast to the wild-type enzyme which shows a catalytic efficiency for 4-nitrophenyl beta-D-mannopyranoside that is 5fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 4fold higher than that for 4-nitrophenyl beta-D-glucopyranoside, the mutant enzyme shows highest catalytic efficiency towards 4-nitrophenyl beta-D-glucopyranoside, 2fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 1.1fold higher than that for 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus horikoshii |
Q77R/D206N | in contrast to the wild-type enzyme which shows a catalytic efficiency for 4-nitrophenyl beta-D-mannopyranoside that is 5fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 4fold higher than that for 4-nitrophenyl beta-D-glucopyranoside, the mutant enzyme shows highest catalytic efficiency towards 4-nitrophenyl beta-D-glucopyranoside, 8fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 1.4fold higher than that for 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus horikoshii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol | - |
Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme D206N | Pyrococcus horikoshii | |
0.44 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus horikoshii | |
0.68 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme Q77R/D206N | Pyrococcus horikoshii | |
1.1 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme Q77R | Pyrococcus horikoshii | |
3.2 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme D206N | Pyrococcus horikoshii | |
7.3 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus horikoshii | |
7.8 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme D206N | Pyrococcus horikoshii | |
13.5 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus horikoshii | |
16.2 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme Q77R | Pyrococcus horikoshii | |
16.8 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme Q77R | Pyrococcus horikoshii | |
30.4 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme Q77R/D206N | Pyrococcus horikoshii | |
36.1 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme Q77R/D206N | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56457 | - |
4 * 56457, calculated from sequence | Pyrococcus horikoshii |
56500 | - |
4 * 56500, SDS-PAGE | Pyrococcus horikoshii |
250000 | - |
gel filtration | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58237 | - |
- |
Pyrococcus horikoshii DSM 12428 | O58237 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-galactopyranoside is 20% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl-beta-D-mannopyranoside | Pyrococcus horikoshii | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-galactopyranoside is 20% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl-beta-D-mannopyranoside | Pyrococcus horikoshii DSM 12428 | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-glucopyranoside is 26% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-mannopyranoside. The catalytic efficiency (kcat/Km) of the mutant enzymes Q77R, D206N and Q77R/D206N for 4-nitrophenyl beta-D-glucopyranoside is higher then that for 4-nitrophenyl beta-D-galactopyranoside and 4-nitrophenyl-beta-D-mannopyranoside | Pyrococcus horikoshii | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-glucopyranoside is 26% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-mannopyranoside. The catalytic efficiency (kcat/Km) of the mutant enzymes Q77R, D206N and Q77R/D206N for 4-nitrophenyl beta-D-glucopyranoside is higher then that for 4-nitrophenyl beta-D-galactopyranoside and 4-nitrophenyl-beta-D-mannopyranoside | Pyrococcus horikoshii DSM 12428 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Pyrococcus horikoshii | 4-nitrophenol + beta-D-mannose | - |
? | |
4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Pyrococcus horikoshii DSM 12428 | 4-nitrophenol + beta-D-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 56500, SDS-PAGE | Pyrococcus horikoshii |
tetramer | 4 * 56457, calculated from sequence | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
BglB | - |
Pyrococcus horikoshii |
PH0501 | locus name | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
101 | - |
melting temperature is 100.7°C | Pyrococcus horikoshii |
102 | - |
half-life: 27 min | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme Q77R | Pyrococcus horikoshii | |
4.2 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus horikoshii | |
6.8 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme Q77R/D206N | Pyrococcus horikoshii | |
13.5 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus horikoshii | |
14.2 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme Q77R | Pyrococcus horikoshii | |
25.5 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme Q77R | Pyrococcus horikoshii | |
34.4 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus horikoshii | |
45 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme D206N | Pyrococcus horikoshii | |
66.5 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme Q77R/D206N | Pyrococcus horikoshii | |
252 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme D206N | Pyrococcus horikoshii | |
439 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme Q77R/D206N | Pyrococcus horikoshii | |
571 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme D206N | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.75 | - |
- |
Pyrococcus horikoshii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000014 | - |
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol | pH 5.0, 90°C, mutant enzyme D206N | Pyrococcus horikoshii | |
0.02 | - |
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol | pH 5.0, 90°C, mutant enzyme Q77R/D206N | Pyrococcus horikoshii | |
0.1 | - |
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol | pH 5.0, 90°C, wild-type enzyme | Pyrococcus horikoshii | |
0.11 | - |
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol | pH 5.0, 90°C, mutant enzyme Q77R | Pyrococcus horikoshii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.85 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme Q77R | Pyrococcus horikoshii | |
1.4 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme Q77R | Pyrococcus horikoshii | |
1.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme Q77R | Pyrococcus horikoshii | |
1.8 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme Q77R/D206N | Pyrococcus horikoshii | |
1.9 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus horikoshii | |
2.58 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus horikoshii | |
9.8 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus horikoshii | |
9.9 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme Q77R/D206N | Pyrococcus horikoshii | |
14.4 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme Q77R/D206N | Pyrococcus horikoshii | |
14.7 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme D206N | Pyrococcus horikoshii | |
75.8 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme D206N | Pyrococcus horikoshii | |
870 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme D206N | Pyrococcus horikoshii |