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Literature summary for 3.2.1.21 extracted from

  • Nakajima, M.; Yoshida, R.; Miyanaga, A.; Abe, K.; Takahashi, Y.; Sugimoto, N.; Toyoizumi, H.; Nakai, H.; Kitaoka, M.; Taguchi, H.
    Functional and structural analysis of a beta-glucosidase involved in beta-1,2-glucan metabolism in Listeria innocua (2016), PLoS ONE, 11, e0148870 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of Lin1840 wild-type and mutant D270A in complexes with sophorose and laminaribiose. Residue Arg572 forms many hydrogen bonds with sophorose and laminaribiose at subsite +1, a key factor for substrate selectivity. The opposite side of subsite +1 from Arg572 is connected to a large empty space, which may be subsite +2 for the binding of sophorotriose Listeria innocua Clip11262

Protein Variants

Protein Variants Comment Organism
D270A nucleophile mutant, crystallization data Listeria innocua Clip11262
R572K mutation in residue important for substrate recognition, 10fold increase in Km value for sophorose Listeria innocua Clip11262

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin competitive Listeria innocua Clip11262
cellobiose mixed type inhibition Listeria innocua Clip11262
D-glucose mixed type inhibition Listeria innocua Clip11262
gentiobiose mixed type inhibition Listeria innocua Clip11262
gluconic acid delta-lactone competitive Listeria innocua Clip11262
isofagomine D-tartrate competitive Listeria innocua Clip11262

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 sophorose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
2.7
-
 laminaribiose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
3.1
-
 4-nitrophenyl-beta-D-glucoside wild-type, pH 6.0, 20°C Listeria innocua Clip11262
3.7
-
 laminaritriose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
5.4
-
 laminaritetraose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
5.5
-
 Laminaripentaose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
9.2
-
 laminaribiose mutant R572K, pH 6.0, 20°C Listeria innocua Clip11262
10
-
 sophorotetraose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
11
-
 sophoropentaose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
15
-
 sophorotriose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
21
-
 sophorose mutant R572K, pH 6.0, 20°C Listeria innocua Clip11262

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 gel filtration Listeria innocua Clip11262

Organism

Organism UniProt Comment Textmining
Listeria innocua Clip11262 Q92AS9
-
-
Listeria innocua Clip11262 ATCC BAA-680 Q92AS9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-beta-D-glucoside + H2O
-
 Listeria innocua Clip11262 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl-beta-D-glucoside + H2O
-
 Listeria innocua Clip11262 ATCC BAA-680 4-nitrophenol + D-glucose
-
 ?
laminaribiose + H2O
-
 Listeria innocua Clip11262 2 D-glucose
-
 ?
laminaribiose + H2O
-
 Listeria innocua Clip11262 ATCC BAA-680 2 D-glucose
-
 ?
laminaripentaose + H2O
-
 Listeria innocua Clip11262 laminaritetraose + D-glucose
-
 ?
laminaritetraose + H2O
-
 Listeria innocua Clip11262 laminaritriose + D-glucose
-
 ?
laminaritriose + H2O
-
 Listeria innocua Clip11262 laminaribiose + D-glucose
-
 ?
laminaritriose + H2O
-
 Listeria innocua Clip11262 ATCC BAA-680 laminaribiose + D-glucose
-
 ?
additional information no substrates: cellobiose, gentiobiose Listeria innocua Clip11262 ?
-
-
additional information no substrates: cellobiose, gentiobiose Listeria innocua Clip11262 ATCC BAA-680 ?
-
-
sophoropentaose + H2O
-
 Listeria innocua Clip11262 sophorotetraose + D-glucose
-
 ?
sophorose + H2O
-
 Listeria innocua Clip11262 2 D-glucose
-
 ?
sophorose + H2O
-
 Listeria innocua Clip11262 ATCC BAA-680 2 D-glucose
-
 ?
sophorotetraose + H2O
-
 Listeria innocua Clip11262 sophorotriose + D-glucose
-
 ?
sophorotriose + H2O
-
 Listeria innocua Clip11262 sophorose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 79466, calculated from sequence Listeria innocua Clip11262

Synonyms

Synonyms Comment Organism
Lin1840
-
 Listeria innocua Clip11262

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Listeria innocua Clip11262

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
 drastic loss of activity above Listeria innocua Clip11262

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 sophoropentaose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
3.5
-
 sophorotetraose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
11
-
 laminaritriose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
12
-
 Laminaripentaose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
14
-
 laminaribiose mutant R572K, pH 6.0, 20°C Listeria innocua Clip11262
14
-
 laminaritetraose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
15
-
 sophorose mutant R572K, pH 6.0, 20°C Listeria innocua Clip11262
23
-
 laminaribiose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
41
-
 sophorose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
48
-
 4-nitrophenyl-beta-D-glucoside wild-type, pH 6.0, 20°C Listeria innocua Clip11262
56
-
 sophorotriose wild-type, pH 6.0, 20°C Listeria innocua Clip11262

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Listeria innocua Clip11262

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
 Listeria innocua Clip11262

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0041
-
 isofagomine D-tartrate wild-type, pH 6.0, 20°C Listeria innocua Clip11262
0.29
-
 1-deoxynojirimycin wild-type, pH 6.0, 20°C Listeria innocua Clip11262
0.3
-
 gluconic acid delta-lactone wild-type, pH 6.0, 20°C Listeria innocua Clip11262
2.8
-
 gentiobiose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
3.8
-
 D-glucose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
21
-
 cellobiose wild-type, pH 6.0, 20°C Listeria innocua Clip11262

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.098
-
 sophoropentaose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
0.34
-
 sophorotetraose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
0.69
-
 sophorose mutant R572K, pH 6.0, 20°C Listeria innocua Clip11262
1.5
-
 laminaribiose mutant R572K, pH 6.0, 20°C Listeria innocua Clip11262
2.1
-
 Laminaripentaose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
2.6
-
 laminaritetraose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
2.9
-
 laminaritriose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
3.8
-
 sophorotriose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
8.8
-
 laminaribiose wild-type, pH 6.0, 20°C Listeria innocua Clip11262
15
-
 4-nitrophenyl-beta-D-glucoside wild-type, pH 6.0, 20°C Listeria innocua Clip11262
21
-
 sophorose wild-type, pH 6.0, 20°C Listeria innocua Clip11262