Application | Comment | Organism |
---|---|---|
biofuel production | biodegradation of lignocellulosic biomass involves a concerted attack by several enzymes, including beta-glucosidases as key component. Current methodologies for biomass conversion to biofuels employ physical and/or chemical pretreatments that disrupt the lignocellulosic biomass in plant cell walls in combination with enzymatic hydrolysis of the cellulose to produce free sugars. Thus, stable cellulolytic enzymes with high enzymatic activity in pretreatment biomass conditions, including high temperatures and acidic conditions, are essential at an industrial scale production. These two features makes beta-glucosidase TpBGL1 to be of significant biotechnological interest | Thermotoga petrophila |
biofuel production | biodegradation of lignocellulosic biomass involves a concerted attack by several enzymes, including beta-glucosidases as key component. Current methodologies for biomass conversion to biofuels employ physical and/or chemical pretreatments that disrupt the lignocellulosic biomass in plant cell walls in combination with enzymatic hydrolysis of the cellulose to produce free sugars. Thus, stable cellulolytic enzymes with high enzymatic activity in pretreatment biomass conditions, including high temperatures and acidic conditions, are essential at an industrial scale production. These two features makes beta-glucosidase TpBGL3 to be of significant biotechnological interest | Thermotoga petrophila |
Cloned (Comment) | Organism |
---|---|
- |
Thermotoga petrophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
beta-glucosidase TpBGL1, SDS-PAGE | Thermotoga petrophila |
84000 | - |
beta-glucosidase TpBGL3, SDS-PAGE | Thermotoga petrophila |
102000 | - |
beta-glucosidase TpBGL3, dynamic light scattering at pH 6 | Thermotoga petrophila |
113000 | - |
beta-glucosidase TpBGL1, dynamic light scattering at pH 4 | Thermotoga petrophila |
167000 | - |
beta-glucosidase TpBGL1, dynamic light scattering at pH 4 | Thermotoga petrophila |
182000 | - |
beta-glucosidase TpBGL3, dynamic light scattering at pH 6 | Thermotoga petrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga petrophila | A5IL43 | - |
- |
Thermotoga petrophila | A5IL97 | - |
- |
Thermotoga petrophila DSM 13995 | A5IL43 | - |
- |
Thermotoga petrophila DSM 13995 | A5IL97 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga petrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila DSM 13995 | 4-nitrophenol + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | beta-glucosidase TpBGL1 is a stable homodimer in solution. The oligomerization state does change in response to decreasing the pH from 6 to 4 at 20°C | Thermotoga petrophila |
homodimer | beta-glucosidase TpBGL3 is a stable homodimer in solution. The oligomerization state does change in response to decreasing the pH from 6 to 4 at 20°C | Thermotoga petrophila |
Synonyms | Comment | Organism |
---|---|---|
TpBGL1 | - |
Thermotoga petrophila |
TpBGL3 | - |
Thermotoga petrophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Thermotoga petrophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
at pH 4 the ellipticity at 222 nm of beta-glucosidase TpBGL1 remains relatively constant at temperatures lower than 80°C. A progressive decrease is observed when the temperature is increased above 80°C | Thermotoga petrophila |
80 | 90 | at temperatures between 80 and 90°C, beta-glucosidase TpBGL1 is more unstable at pH 4 than pH 6 | Thermotoga petrophila |
90 | - |
for beta-glucosidase TpBGL3 at both pH 6 and 4, the ellipticities at 222 nm remain relatively constant at temperatures lower than 90°C. A decrease in the ellipticities at 222 nm is observed at temperatures higher than 90°C, suggesting a loss of regular secondary structure, the process is irreversible | Thermotoga petrophila |
100 | - |
beta-glucosidase TpBGL1 at pH 6 can not be completely unfolded at 100°C. At pH 4 TpBGL1 drastically loses regular secondary structure at temperatures higher than 80°C with a complete unfolding at 100°C. At both pH values, the processes are essentially irreversible | Thermotoga petrophila |
100 | - |
beta-glucosidase TpBGL3 can be thermally denatured at pH 4 and at pH 6, the process is irreversible | Thermotoga petrophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
beta-glucosidase TpBGL3 | Thermotoga petrophila |
6 | - |
beta-glucosidase TpBGL1 | Thermotoga petrophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | pH 3.0: about 65% of maximal activity, pH 5.0: about 70% of maximal activity, beta-glucosidase TpBGL3 | Thermotoga petrophila |
4 | 8 | pH 4.0: about 45% of maximal activity, pH 8.0: about 60% of maximal activity, beta-glucosidase TpBGL1 | Thermotoga petrophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
beta-glucosidase TpBGL1 shows higher stability at pH 6 than at pH 4 | Thermotoga petrophila |