Application | Comment | Organism |
---|---|---|
synthesis | optimized medium composition for beta-glucosidase expression is corn cob (51.8 g/l), beef extract (23.8 g/l), salicin (0.5 g/l), MnSO4·H2O (0.363 g/l), MgSO4·7H2O (0.4 g/l), and NaCl (5 g/l). Under the optimal conditions, the activity of beta-glucosidase is up to 4.71 U/ml | Bacillus sp. ZJ1308 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 5 mM, 19% residual activity | Bacillus sp. ZJ1308 | |
Ca2+ | 5 mM, 68% residual activity | Bacillus sp. ZJ1308 | |
Co2+ | 5 mM, 58% residual activity | Bacillus sp. ZJ1308 | |
Cu2+ | 5 mM, 48% residual activity | Bacillus sp. ZJ1308 | |
K+ | 5 mM, 62% residual activity | Bacillus sp. ZJ1308 | |
Mg2+ | 5 mM, 72% residual activity | Bacillus sp. ZJ1308 | |
Mn2+ | 5 mM, 83% residual activity | Bacillus sp. ZJ1308 | |
Ni2+ | 5 mM, 21% residual activity | Bacillus sp. ZJ1308 | |
Zn2+ | 5 mM, 63% residual activity | Bacillus sp. ZJ1308 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Bacillus sp. ZJ1308 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. ZJ1308 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. ZJ1308 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
110.5 | - |
pH 7.0, 50°C | Bacillus sp. ZJ1308 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Bacillus sp. ZJ1308 | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Bacillus sp. ZJ1308 | 4-nitrophenol + beta-D-glucose | - |
? | |
salicin + H2O | - |
Bacillus sp. ZJ1308 | saligenin + beta-D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. ZJ1308 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
30 min, 56% residual activity | Bacillus sp. ZJ1308 |
90 | - |
30 min, 38% residual activity | Bacillus sp. ZJ1308 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus sp. ZJ1308 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Bacillus sp. ZJ1308 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
700 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 7.0, 50°C | Bacillus sp. ZJ1308 |