Cloned (Comment) | Organism |
---|---|
heterogeneous expression in Pichia pastoris strain GS115 | Humicola insolens |
Protein Variants | Comment | Organism |
---|---|---|
F304I | mutant enzyme shows no activity with 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
I278F | decreased activities towards 4-nitrophenyl beta-D-glucopyranoside, hydrolysis activity on sophorose, which is much higher than the activity on 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
I48W | decreased activities towards 4-nitrophenyl beta-D-glucopyranoside, hydrolysis activity on sophorose, which is much higher than the activity on 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
I48W/I278F/T484Y | decreased activities towards 4-nitrophenyl beta-D-glucopyranoside, hydrolysis activity on sophorose, which is much higher than the activity on 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
T484Y | decreased activities towards 4-nitrophenyl beta-D-glucopyranoside, hydrolysis activity on sophorose, which is much higher than the activity on 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
W69I | mutant enzyme shows no activity with 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
Y509T | introduction of mutation Y509T causes the increase of the Km value, but does not affect the kcat value | Humicola insolens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, 22% inhibition | Humicola insolens | |
Ag+ | 5 mM, 72% inhibition; 5 mM, 76% inhibition; 5 mM, 89% inhibition | Humicola insolens | |
Co2+ | 5 mM, 10% inhibition | Humicola insolens | |
Cr3+ | 5 mM, 30% inhibition | Humicola insolens | |
Cu2+ | 5 mM, 6% inhibition | Humicola insolens | |
D-glucose | - |
Humicola insolens | |
EDTA | 5 mM, 9% inhibition | Humicola insolens | |
Fe3+ | 5 mM, 5% inhibition; 5 mM, 6% inhibition; 5 mM, 7% inhibition | Humicola insolens | |
Mg2+ | 5 mM, 23% loss of activity | Humicola insolens | |
Ni2+ | 5 mM, 10% inhibition | Humicola insolens | |
Pb2+ | 5 mM, 14% inhibition | Humicola insolens | |
SDS | 5 mM, 55% inhibition; 5 mM, 62% inhibition; 5 mM, 95% inhibition | Humicola insolens | |
Zn2+ | 5 mM, 10% loss of activity | Humicola insolens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 60°C | Humicola insolens | |
0.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 60°C, wild-type enzyme | Humicola insolens | |
1.51 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, wild-type enzyme | Humicola insolens | |
1.99 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme I48W | Humicola insolens | |
2.37 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme T484Y | Humicola insolens | |
2.42 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme I278F | Humicola insolens | |
2.45 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 60°C, mutant enzyme Y509T | Humicola insolens | |
2.48 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme I48W/I278F/T484Y | Humicola insolens | |
6.63 | - |
cellobiose | pH 5.5, 55°C | Humicola insolens | |
8.44 | - |
cellobiose | pH 5.5, 60°C, wild-type enzyme | Humicola insolens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM, 1.1fold activiation | Humicola insolens | |
Mn2+ | 5 mM, 1.2fold activiation | Humicola insolens | |
Mn2+ | 5 mM, 1.3fold activiation | Humicola insolens | |
additional information | no remarkable activation or inhibition by 5 mM Mg2+, 5 mM Zn2+, 5 mM Ca2+, 5 mM Cu2+, 5 mM Co2+, 5 mM Cr2+, 5 mM Ni2+, 5 mM Pb2+, 5 mM EDTA, 5 mM 2-mercaptoethanol | Humicola insolens | |
Zn2+ | 5 mM, 1.1fold activiation | Humicola insolens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78280 | - |
calculated from sequence | Humicola insolens |
80000 | - |
- |
Humicola insolens |
96870 | - |
calculated from sequence | Humicola insolens |
97120 | - |
calculated from sequence | Humicola insolens |
120000 | - |
1 * 120000, SDS-PAGE | Humicola insolens |
127000 | - |
- |
Humicola insolens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humicola insolens | A0A191KCR0 | - |
- |
Humicola insolens | A0A191KCR1 | - |
- |
Humicola insolens | A0A191KCR2 | - |
- |
Humicola insolens Y1 | A0A191KCR0 | - |
- |
Humicola insolens Y1 | A0A191KCR1 | - |
- |
Humicola insolens Y1 | A0A191KCR2 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 12 putative N-glycosylation sites | Humicola insolens |
glycoprotein | 2 putative N-glycosylation sites | Humicola insolens |
glycoprotein | 7 putative N-glycosylation sites | Humicola insolens |
Purification (Comment) | Organism |
---|---|
- |
Humicola insolens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.95 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 50°C, mutant enzyme I48W | Humicola insolens |
1 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 50°C, mutant enzyme I48W/I278F/T484Y | Humicola insolens |
2.68 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 50°C, mutant enzyme I278F | Humicola insolens |
3.36 | - |
substrate: sophorose, pH 6.0, 50°C, mutant enzyme I48W/I278F/T484Y | Humicola insolens |
3.59 | - |
substrate: sophorose, pH 6.0, 50°C, mutant enzyme I278F | Humicola insolens |
3.7 | - |
substrate: sophorose, pH 6.0, 50°C, mutant enzyme I48W | Humicola insolens |
4.46 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 50°C, mutant enzyme T484Y | Humicola insolens |
5.64 | - |
substrate: sophorose, pH 6.0, 50°C, mutant enzyme T484Y | Humicola insolens |
22.3 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 5.5, 60°C, mutant enzyme Y509T | Humicola insolens |
31.6 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 50°C, wild-type enzyme | Humicola insolens |
36.3 | - |
substrate: substrate: cellobiose, pH 5.5, 60°C, wild-type enzyme | Humicola insolens |
56.4 | - |
substrate: cellobiose, pH 5.5, 55°C | Humicola insolens |
57.5 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 5.5, 60°C, wild-type enzyme | Humicola insolens |
62.6 | - |
substrate: daidzin, pH 6.0, 50°C | Humicola insolens |
89 | - |
substrate: daidzin, pH 5.5, 55°C | Humicola insolens |
94.6 | - |
substrate: sophorose, pH 5.5, 60°C, wild-type enzyme | Humicola insolens |
103.4 | - |
substrate: gentiobiose, pH 5.5, 60°C, wild-type enzyme | Humicola insolens |
113.9 | - |
substrate: sophorose, pH 5.5, 55°C | Humicola insolens |
158.8 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 5.5, 60°C | Humicola insolens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Humicola insolens | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Humicola insolens | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Humicola insolens Y1 | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Humicola insolens | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Humicola insolens Y1 | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Humicola insolens | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Humicola insolens Y1 | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Humicola insolens | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Humicola insolens Y1 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
cellobiose + H2O | - |
Humicola insolens | 2 D-glucose | - |
? | |
cellobiose + H2O | - |
Humicola insolens Y1 | 2 D-glucose | - |
? | |
daidzin + H2O | - |
Humicola insolens | beta-D-glucose + daidzein | - |
? | |
daidzin + H2O | - |
Humicola insolens Y1 | beta-D-glucose + daidzein | - |
? | |
genistin + H2O | - |
Humicola insolens | beta-D-glucose + genistein | - |
? | |
gentiobiose + H2O | - |
Humicola insolens | D-glucose | - |
? | |
gentiobiose + H2O | - |
Humicola insolens Y1 | D-glucose | - |
? | |
glycitin + H2O | - |
Humicola insolens | beta-D-glucose + glycitein | - |
? | |
sophorose + H2O | - |
Humicola insolens | 2 D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 120000, SDS-PAGE | Humicola insolens |
monomer | 1 * 127000, calculated from sequence | Humicola insolens |
monomer | 1 * 78276, calculated from sequence | Humicola insolens |
monomer | 1 * 80000, calculated from sequence | Humicola insolens |
monomer | 1 * 96873, calculated from sequence | Humicola insolens |
monomer | 1 * 97117, calculated from sequence | Humicola insolens |
Synonyms | Comment | Organism |
---|---|---|
HiBgl3A | - |
Humicola insolens |
HiBgl3B | - |
Humicola insolens |
HiBgl3C | - |
Humicola insolens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | with 4-nitrophenyl beta-D-glucopyranoside as substrate | Humicola insolens |
55 | - |
with 4-nitrophenyl beta-D-glucopyranoside as substrate | Humicola insolens |
60 | - |
with 4-nitrophenyl beta-D-glucopyranoside as substrate | Humicola insolens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | 40°C: about 50% of maximal activity, 60°C: about 85% of maximal activity, substrate: 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
40 | 60 | 40°C: about 65% of maximal activity, 60°C: about 70% of maximal activity, substrate: 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
50 | 65 | 50°C: about 45% of maximal activity, 65°C: about 50% of maximal activity, substrate: 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
1 h, no loss of activity | Humicola insolens |
50 | - |
1 h, about 10% loss of activity | Humicola insolens |
60 | - |
1 h, about 55% loss of activity | Humicola insolens |
60 | - |
30 min, about 90% loss of activity | Humicola insolens |
70 | - |
1 h, 10 min, 95% loss of activity | Humicola insolens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.21 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme I48W | Humicola insolens | |
4.24 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme I48W/I278F/T484Y | Humicola insolens | |
9.61 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme I278F | Humicola insolens | |
15.05 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme T484Y | Humicola insolens | |
40.42 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, wild-type enzyme | Humicola insolens | |
68.16 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 60°C, mutant enzyme Y509T | Humicola insolens | |
73.08 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 60°C, wild-type enzyme | Humicola insolens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | with 4-nitrophenyl beta-D-glucopyranoside as substrate | Humicola insolens |
5.5 | - |
with 4-nitrophenyl beta-D-glucopyranoside as substrate | Humicola insolens |
6 | - |
with 4-nitrophenyl beta-D-glucopyranoside as substrate | Humicola insolens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | pH 4.0: about 30% of maximal activity, pH 8.0: about 30% of maximal activity, substrate: 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
5 | 6.5 | pH 5.0: about 30% of maximal activity, pH 6.5: about 60% of maximal activity, substrate: 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
5 | 6.5 | pH 5.0: about 90% of maximal activity, pH 6.5: about 70% of maximal activity, substrate: 4-nitrophenyl beta-D-glucopyranoside | Humicola insolens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 37°C, 1 h, enzyme retains more than 80% activity | Humicola insolens |
5 | 10 | 37°C, 1 h, enzyme retains more than 80% activity | Humicola insolens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 5 | D-glucose | pH 5.5, 60°C, with 4-nitrophenyl beta-D-glucopyranoside as substrate, wild-type enzyme | Humicola insolens | |
37.1 | - |
D-glucose | pH 5.5, 55°C, with 4-nitrophenyl beta-D-glucopyranoside as substrate | Humicola insolens | |
55.2 | - |
D-glucose | pH 6.0, 50°C, with 4-nitrophenyl beta-D-glucopyranoside as substrat, wild-type enzyme | Humicola insolens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Humicola insolens | calculated | - |
5.93 |
Humicola insolens | calculated | - |
6.03 |
Humicola insolens | calculated | - |
6.26 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.61 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme I48W | Humicola insolens | |
1.71 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme I48W/I278F/T484Y | Humicola insolens | |
3.98 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme I278F | Humicola insolens | |
6.36 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, mutant enzyme T484Y | Humicola insolens | |
11.1 | - |
cellobiose | pH 5.5, 60°C, wild-type enzyme | Humicola insolens | |
23 | - |
cellobiose | pH 5.5, 55°C | Humicola insolens | |
26.77 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, wild-type enzyme | Humicola insolens | |
27.82 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 60°C, mutant enzyme Y509T | Humicola insolens | |
81.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 60°C, wild-type enzyme | Humicola insolens | |
1557 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 60°C | Humicola insolens |