Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slight inhibition | Paecilomyces sp. 'thermophila' | |
Ag+ | 80% inhibition at 4 mM | Paecilomyces sp. 'thermophila' | |
D-glucono-1,5-lactone | competitive inhibition | Paecilomyces sp. 'thermophila' | |
D-glucose | competitive inhibition | Paecilomyces sp. 'thermophila' | |
EDTA | slight inhibition | Paecilomyces sp. 'thermophila' | |
Hg2+ | 95.5% inhibition at 4 mM | Paecilomyces sp. 'thermophila' | |
additional information | no or poor inhibition by Ca2+ and DTT | Paecilomyces sp. 'thermophila' | |
SDS | slight inhibition | Paecilomyces sp. 'thermophila' |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
0.65 | - |
cellobiose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
0.77 | - |
gentiobiose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
1.06 | - |
sophorose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
1.39 | - |
Salicin | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
1.45 | - |
amygdalin | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Paecilomyces sp. 'thermophila' | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Paecilomyces sp. 'thermophila' | |
Cu2+ | activates slightly | Paecilomyces sp. 'thermophila' | |
Fe2+ | activates by 36% | Paecilomyces sp. 'thermophila' | |
Mg2+ | activates | Paecilomyces sp. 'thermophila' | |
Mn2+ | activates | Paecilomyces sp. 'thermophila' | |
additional information | no metalloprotein, not affected by Al3+ at 1.0 mM | Paecilomyces sp. 'thermophila' | |
Ni2+ | activates | Paecilomyces sp. 'thermophila' | |
Sr2+ | activates slightly | Paecilomyces sp. 'thermophila' | |
Zn2+ | activates | Paecilomyces sp. 'thermophila' |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paecilomyces sp. 'thermophila' | - |
- |
- |
Paecilomyces sp. 'thermophila' J18 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the extracellular enzyme contains 82.3% carbohydrate | Paecilomyces sp. 'thermophila' |
Purification (Comment) | Organism |
---|---|
native enzyme 105fold to homogeneity by anion exchange chromatography and gel filtration | Paecilomyces sp. 'thermophila' |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
97.2 | - |
purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside | Paecilomyces sp. 'thermophila' |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Paecilomyces sp. 'thermophila' | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Paecilomyces sp. 'thermophila' J18 | 4-nitrophenol + beta-D-glucose | - |
? | |
amygdalin + 2 H2O | - |
Paecilomyces sp. 'thermophila' | mandelonitrile + 2 beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Paecilomyces sp. 'thermophila' | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Paecilomyces sp. 'thermophila' J18 | 2 beta-D-glucose | - |
? | |
cellopentaose + H2O | - |
Paecilomyces sp. 'thermophila' | 5 D-glucose | - |
? | |
cellotetraose + 3 H2O | - |
Paecilomyces sp. 'thermophila' | 4 beta-D-glucose | - |
? | |
cellotriose + H2O | - |
Paecilomyces sp. 'thermophila' | 3 D-glucose | - |
? | |
daidzin + H2O | - |
Paecilomyces sp. 'thermophila' | beta-D-glucose + daidzein | - |
? | |
genistin + H2O | - |
Paecilomyces sp. 'thermophila' | beta-D-glucose + genistein | - |
? | |
gentiobiose + H2O | - |
Paecilomyces sp. 'thermophila' | 2 beta-D-glucose | - |
? | |
gentiobiose + H2O | - |
Paecilomyces sp. 'thermophila' J18 | 2 beta-D-glucose | - |
? | |
laminarin + H2O | - |
Paecilomyces sp. 'thermophila' | beta-D-glucose | - |
? | |
lichenan + H2O | - |
Paecilomyces sp. 'thermophila' | beta-D-glucose | - |
? | |
additional information | the extracellular enzyme exhibits a broad substrate specificity, shows also exoglucanase and transglycosylation activities, overview | Paecilomyces sp. 'thermophila' | ? | - |
? | |
additional information | the extracellular enzyme exhibits a broad substrate specificity, shows also exoglucanase and transglycosylation activities, overview | Paecilomyces sp. 'thermophila' J18 | ? | - |
? | |
salicin + H2O | - |
Paecilomyces sp. 'thermophila' | beta-D-glucose + 2-(hydroxymethyl)phenol | - |
? | |
sophorose + H2O | - |
Paecilomyces sp. 'thermophila' | 2 beta-D-glucose | - |
? | |
sophorose + H2O | - |
Paecilomyces sp. 'thermophila' J18 | 2 beta-D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Paecilomyces sp. 'thermophila' |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature profile | Paecilomyces sp. 'thermophila' |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
purified enzyme, quite stable up to | Paecilomyces sp. 'thermophila' |
70 | - |
purified enzyme, 57.9% remaining activity | Paecilomyces sp. 'thermophila' |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
sophorose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
4.2 | - |
gentiobiose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
4.5 | - |
cellobiose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
7.1 | - |
Salicin | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
7.9 | - |
amygdalin | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
13 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Paecilomyces sp. 'thermophila' |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | pH profile | Paecilomyces sp. 'thermophila' |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
D-glucono-1,5-lactone | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' | |
73 | - |
D-glucose | pH 6.2, 50°C | Paecilomyces sp. 'thermophila' |