General Stability | Organism |
---|---|
presence of CHAPS necessary for maximum activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Saccharomyces cerevisiae | |
EGTA | complete inhibition at 5 mM | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Man9GlcNAc | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | from microsomal membranes | Saccharomyces cerevisiae | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibition by EGTA can be 90% restored after addition of 0.1 mM | Saccharomyces cerevisiae | |
Zn2+ | 30% stimulation of the EGTA-inhibited enzyme at 0.1 mM | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 44000 + 1 * 23000, reducing SDS-PAGE | Saccharomyces cerevisiae |
44000 | - |
1 * 44000 + 1 * 23000, reducing SDS-PAGE | Saccharomyces cerevisiae |
60000 | 68000 | gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2 + H2O | Saccharomyces cerevisiae | from oligosaccharide-lipid | alpha-D-mannopyranose + ? | - |
? | |
Man7GlcNAc2 + H2O | Saccharomyces cerevisiae | from oligosaccharide-lipid, very low activity | alpha-D-mannopyranose + ? | - |
? | |
Man9GlcNAc2 + H2O | Saccharomyces cerevisiae | from thyroglobulin or oligosaccharide-lipid, trimming the substrate to Man8GlcNAc2, the substrate for oligosaccharide elongation | Man5GlcNAc2 + Man6GlcNAc2 + Man7GlcNAc2 + Man8GlcNAc2 + alpha-D-mannopyranose | only alpha-(1-2)-linked terminal mannoses on the alpha-3-branch of the Man9GlcNAc precursor dispensable | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Saccharomyces cerevisiae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2 + H2O | from oligosaccharide-lipid | Saccharomyces cerevisiae | alpha-D-mannopyranose + ? | - |
? | |
Man7GlcNAc2 + H2O | from oligosaccharide-lipid, very low activity | Saccharomyces cerevisiae | alpha-D-mannopyranose + ? | - |
? | |
Man9GlcNAc + H2O | - |
Saccharomyces cerevisiae | Man8GlcNAc + alpha-D-mannopyranose | - |
? | |
Man9GlcNAc2 + H2O | from thyroglobulin or oligosaccharide-lipid, trimming the substrate to Man8GlcNAc2, the substrate for oligosaccharide elongation | Saccharomyces cerevisiae | Man5GlcNAc2 + Man6GlcNAc2 + Man7GlcNAc2 + Man8GlcNAc2 + alpha-D-mannopyranose | only alpha-(1-2)-linked terminal mannoses on the alpha-3-branch of the Man9GlcNAc precursor dispensable | ? | |
p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Saccharomyces cerevisiae | p-nitrophenol + alpha-D-mannopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 44000 + 1 * 23000, reducing SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Man9-alpha-mannosidase | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 6.8 | activity falls rapid below and above | Saccharomyces cerevisiae |