Literature summary for 3.2.1.208 extracted from

Nobre, A.; Empadinhas, N.; Nobre, M.; Lourenco, E.; Maycock, C.; Ventura, M.; Mingote, A.; da Costa, M.
The plant Selaginella moellendorffii possesses enzymes for synthesis and hydrolysis of the compatible solutes mannosylglycerate and glucosylglycerate (2013), Planta, 237, 891-901 .

Search for more literature for 3.2.1.208

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Selaginella moellendorffii

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibitory: EDTA	Selaginella moellendorffii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.9	-	2-O-(alpha-D-glucopyranosyl)-D-glycerate	pH 6.0, 25°C	Selaginella moellendorffii
5.9	-	2-O-(alpha-D-glucopyranosyl)-D-glycerate	pH 6.0, 40°C	Selaginella moellendorffii
11.8	-	2-O-(alpha-D-mannopyranosyl)-D-glycerate	pH 6.0, 40°C	Selaginella moellendorffii
21.4	-	2-O-(alpha-D-mannopyranosyl)-D-glycerate	pH 6.0, 25°C	Selaginella moellendorffii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activation by 10 mM cation in decreasing order of efficiency is Mn2+, Ca2+, Mg2+	Selaginella moellendorffii
Mg2+	activation by 10 mM cation in decreasing order of efficiency is Mn2+, Ca2+, Mg2+	Selaginella moellendorffii
Mn2+	maximum activity in presence of 10 mM Mn2+. Activation by 10 mM cation in decreasing order of efficiency is Mn2+, Ca2+, Mg2+	Selaginella moellendorffii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55300	-	gel filtration	Selaginella moellendorffii

Organism

Organism	UniProt	Comment	Textmining
Selaginella moellendorffii	D8QTR2	-	-

Storage Stability

Storage Stability	Organism
0°C, stable for more than 2 months, stable upon freeze/Thawing	Selaginella moellendorffii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-O-(alpha-D-glucopyranosyl)-D-glycerate + H2O	-	Selaginella moellendorffii	D-glucopyranose + D-glycerate	-	ir
2-O-(alpha-D-mannopyranosyl)-D-glycerate + H2O	-	Selaginella moellendorffii	D-mannopyranose + D-glycerate	-	ir

Subunits

Subunits	Comment	Organism
monomer	1 * 54500, calculated from sequence	Selaginella moellendorffii

Synonyms

Synonyms	Comment	Organism
SELMODRAFT_437793	-	Selaginella moellendorffii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Selaginella moellendorffii

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	60	-	Selaginella moellendorffii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	6.5	-	Selaginella moellendorffii

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)