Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Schizosaccharomyces pombe |
Crystallization (Comment) | Organism |
---|---|
mannose 6-phosphate receptor homology domain of GII beta subunit bound to mannose, to 1.6 A resolution. No major difference in the overall fold of ligand-bound and unbound structures, but a repositioning of side chains throughout the binding pocket, including Y372 | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutations of the primary binding pocket residues and adjacent W409, all inhibit the activity of GII both in vitro and in vivo, they do not cause a significant change in the overall fold of the GII beta subunit mannose 6-phosphate receptor homology domain but impact locally the stability of the binding pocket | Schizosaccharomyces pombe |
W409A | mutation substantially decreases GII activity | Schizosaccharomyces pombe |
Y372A | mutation of Y372 substantially decreases GII activity | Schizosaccharomyces pombe |
Y372F | mutation of Y372 substantially decreases GII activity | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | Q9USH8 | glucosidase 2 subunit beta | - |
Schizosaccharomyces pombe 972 | Q9USH8 | glucosidase 2 subunit beta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GlcMan9GlcNAc + H2O | - |
Schizosaccharomyces pombe | D-glucose + Man9GlcNAc | - |
? | |
GlcMan9GlcNAc + H2O | - |
Schizosaccharomyces pombe 972 | D-glucose + Man9GlcNAc | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GTB1 | - |
Schizosaccharomyces pombe |