Cloned (Comment) | Organism |
---|---|
expressed with a baculovirus expression system in Sf9 cells, expressed in Cos7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D542N | D542 required for catalytic activity | Homo sapiens |
D564N | D564 required for catalytic activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
castanospermine | - |
Homo sapiens | |
deoxynojirimycin | competitive, strong inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
p-nitrophenyl-alpha-D-glucopyranoside | pH 7.5, 37°C, alpha1-beta complex | Homo sapiens | |
0.52 | - |
p-nitrophenyl-alpha-D-glucopyranoside | pH 7.5, 37°C, alpha2-beta complex | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
SDS-PAGE, beta subunit | Homo sapiens |
110000 | - |
SDS-PAGE, alpha1 subunit | Homo sapiens |
250000 | - |
native PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc2Man9GlcNAc2 + H2O | Homo sapiens | involved in the Calnexin Cycle | Man9GlcNAc2 + alpha-D-glucose | - |
? | |
additional information | Homo sapiens | involved in early glycoprotein biogenesis, catalyzes the hydrolysis of two alpha-1,3-linked glucose residues present on all Asn-linked precursor oligosaccharides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14314 | - |
- |
Homo sapiens | P14314 | beta subunit | - |
Homo sapiens | Q14697 | - |
- |
Homo sapiens | Q14697 | alpha1 isofrom of the alpha subunit, isoforms are formed by alternative splicing | - |
Homo sapiens | Q14697 | alpha2 isoform of the alpha subunit, contains a 66 bp insert, isoforms are formed by alternative splicing | - |
Purification (Comment) | Organism |
---|---|
purification of recombinant protein using His-tag | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Homo sapiens | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Homo sapiens | 4-nitrophenol + D-glucopyranose | - |
? | |
Glc2Man9GlcNAc2 + H2O | involved in the Calnexin Cycle | Homo sapiens | Man9GlcNAc2 + alpha-D-glucose | - |
? | |
additional information | involved in early glycoprotein biogenesis, catalyzes the hydrolysis of two alpha-1,3-linked glucose residues present on all Asn-linked precursor oligosaccharides | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha, beta, two different isoforms of the alpha subunit identified, alpha1 is the short and alpha2 the long isoform, both isoforms form dimers with the beta subunit, only dimeric form shows catalytic activity | Homo sapiens |
dimer | alpha, beta, two different isoforms of the alpha subunit identified, alpha1 is the short and alpha2 the long isoforms, both isoforms forms dimers with the beta subunit, only dimeric form shows catalytic activity | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
deoxynojirimycin | pH 7.5, 37°C | Homo sapiens | |
0.015 | - |
castanospermine | pH 7.5, 37°C | Homo sapiens | |
0.017 | - |
castanospermine | pH 7.5, 37°C | Homo sapiens |