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Literature summary for 3.2.1.203 extracted from
- Rationally selected single-site mutants of the Thermoascus aurantiacus endoglucanase increase hydrolytic activity on cellulosic substrates (2012), Biotechnol. Bioeng., 109, 1595-1599 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Thermoascus aurantiacus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F16S | 1.7fold increase in kcat and 1.5fold improvement in hydrolytic activity on cellulosic substrates, while maintaining thermostability | Thermoascus aurantiacus |
| F16S/Y95F | 60% reduction in catalytic efficiency | Thermoascus aurantiacus |
| Y95F | 4.0fold increase in kcat and 2.5fold improvement in hydrolytic activity on cellulosic substrates, while maintaining thermostability | Thermoascus aurantiacus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carboxymethyl cellulose | Km value is 0.14 mg/ml, 0.17 mg/ml, 0.13 mg/ml, and 0.17 mg/ml for wild-type, mutant F16S, mutant Y95F and mutant F16S/Y95F, respectively | Thermoascus aurantiacus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoascus aurantiacus | Q96UI5 | - |
- |
| Thermoascus aurantiacus IFO 9748 | Q96UI5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Thermoascus aurantiacus | carboxymethyl cellulose with endohydrolysed (1->4)-beta-D-glucosidic linkages | - |
? |  |
| carboxymethyl cellulose + H2O | - |
Thermoascus aurantiacus IFO 9748 | carboxymethyl cellulose with endohydrolysed (1->4)-beta-D-glucosidic linkages | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EG1 | - |
Thermoascus aurantiacus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
wild-type and mutants F16S and Y95F | Thermoascus aurantiacus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
wild-type and mutants F16S and Y95F, active for more than 12 h | Thermoascus aurantiacus |
| 70 | - |
wild-type and mutants F16S and Y95F, 2 h, more than 80% residual activity | Thermoascus aurantiacus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.95 | - |
carboxymethyl cellulose | mutant F16S/Y95F, pH 5.0, 70°C | Thermoascus aurantiacus | |
| 3.98 | - |
carboxymethyl cellulose | wild-type, pH 5.0, 70°C | Thermoascus aurantiacus | |
| 7.98 | - |
carboxymethyl cellulose | mutant F16S, pH 5.0, 70°C | Thermoascus aurantiacus | |
| 16.58 | - |
carboxymethyl cellulose | mutant Y95F, pH 5.0, 70°C | Thermoascus aurantiacus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
wild-type and mutants F16S and Y95F | Thermoascus aurantiacus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | wild-type and mutants F16S and Y95F | Thermoascus aurantiacus |
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