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Literature summary for 3.2.1.200 extracted from
- Purification and characterization of a novel chitinase from Trichosanthes dioica seed with antifungal activity (2016), Int. J. Biol. Macromol., 84, 62-68 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5% loss of activity at 20 mM | Trichosanthes dioica |  |
| EDTA | 15% loss of activity at 20 mM | Trichosanthes dioica | |
| Urea | 20% loss of activity at 8 M | Trichosanthes dioica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | enzyme activity increases significantly in the presence of 20 mM Mg2+ | Trichosanthes dioica | |
| Mn2+ | enzyme activity increases significantly in the presence of 20 mM Mn2+ | Trichosanthes dioica | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichosanthes dioica | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Trichosanthes dioica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| QA-cellulose column chromatography | Trichosanthes dioica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Trichosanthes dioica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| colloidal chitin + H2O | - |
Trichosanthes dioica | N-acetyl D-glucosamine + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39000, SDS-PAGE | Trichosanthes dioica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TdsC | - |
Trichosanthes dioica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Trichosanthes dioica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | more than 90% activity between 30 and 80°C | Trichosanthes dioica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
the enzyme retains 90% activity after 30 min at 90°C | Trichosanthes dioica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Trichosanthes dioica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | about 50% activity at pH 4.0, 100% activity at pH 5.0-8.0, about 90% activity at pH 9.0-10.0 | Trichosanthes dioica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | the enzyme remains stable after 30 min at pH 5.0-10.0, 80% activity is left after 30 min at pH 4.0 and 11.0 | Trichosanthes dioica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme shows antifungal activity against Aspergillus niger and Trichoderma sp. | Trichosanthes dioica |
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Release 2023.1 (January 2023)
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