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Literature summary for 3.2.1.200 extracted from

  • Shehata, A.; Abd El Aty, A.; Darwish, D.; Abdel Wahab, W.; Mostafa, F.
    Purification, physicochemical and thermodynamic studies of antifungal chitinase with production of bioactive chitosan-oligosaccharide from newly isolated Aspergillus griseoaurantiacus KX010988 (2018), Int. J. Biol. Macromol., 107, 990-999 .
    View publication on PubMed
Search for more literature for 3.2.1.200

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 63.52% residual activity at 1 mM Aspergillus griseoaurantiacus
Cu2+ complete inhibition at 1 mM Aspergillus griseoaurantiacus
Fe2+ complete inhibition at 1 mM Aspergillus griseoaurantiacus
K+ 10% residual activity at 1 mM Aspergillus griseoaurantiacus
Mg2+ 95% residual activity at 1 mM Aspergillus griseoaurantiacus
Na+ 15% residual activity at 1 mM Aspergillus griseoaurantiacus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 120% activity at 1 mM Aspergillus griseoaurantiacus
Mn2+ 150% activity at 1 mM Aspergillus griseoaurantiacus
Zn2+ 141.8% activity at 1 mM Aspergillus griseoaurantiacus

Organism

Organism UniProt Comment Textmining
Aspergillus griseoaurantiacus
-
-
-
Aspergillus griseoaurantiacus KX010988
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.47
-
 culture filtrate, at pH 4.5 and 40°C Aspergillus griseoaurantiacus
93.75
-
 after 12.55fold purification, at pH 4.5 and 40°C Aspergillus griseoaurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bean peel + H2O
-
 Aspergillus griseoaurantiacus ?
-
 ?
bean peel + H2O
-
 Aspergillus griseoaurantiacus KX010988 ?
-
 ?
colloidal chitin + H2O 100% activity Aspergillus griseoaurantiacus ?
-
 ?
colloidal chitin + H2O 100% activity Aspergillus griseoaurantiacus KX010988 ?
-
 ?
high molecular weight chitin + H2O 90% activity compared to colloidal chitin Aspergillus griseoaurantiacus ?
-
 ?
high molecular weight chitin + H2O 90% activity compared to colloidal chitin Aspergillus griseoaurantiacus KX010988 ?
-
 ?
moderate molecular weight chitin + H2O 40% activity compared to colloidal chitin Aspergillus griseoaurantiacus ?
-
 ?
moderate molecular weight chitin + H2O 40% activity compared to colloidal chitin Aspergillus griseoaurantiacus KX010988 ?
-
 ?
additional information no activity with low molecular weight chitin. The enzyme is not active on rice bran, rice straw, corn stalks, corn leaves and bagasse Aspergillus griseoaurantiacus ?
-
 ?
additional information no activity with low molecular weight chitin. The enzyme is not active on rice bran, rice straw, corn stalks, corn leaves and bagasse Aspergillus griseoaurantiacus KX010988 ?
-
 ?
potato shell + H2O
-
 Aspergillus griseoaurantiacus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 130000, SDS-PAGE Aspergillus griseoaurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Aspergillus griseoaurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the enzyme is stable at 40°C for 15 min. The enzyme loses 40% of its activity when heated at 60°C for 60 min Aspergillus griseoaurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Aspergillus griseoaurantiacus